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NEB/Monarch? DNA Gel Extraction Kit/T1020S/250 preps
  • NEB/Monarch? DNA Gel Extraction Kit/T1020S/250 preps

NEB/Monarch? DNA Gel Extraction Kit/T1020S/250 preps

價(jià)格: ￥5460.00 市場(chǎng)價(jià): 9100.00
貨號(hào): T1020S-250preps
品牌: NEB
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    • Description:

      ?
      TheMonarchDNAGelExtractionKitrapidlyandreliablypurifiesupto5μgofconcentrated,high-qualityDNAfromagarosegels.Thekitutilizesabind/wash/eluteworkflowwithminimalincubationandspintimes.Thecolumnsensurezerobufferretentionandnocarryoverofcontaminants,enablingelutionofsampleinvolumesaslowas6μl.Thebuffersprovidedhavebeenoptimized,anddonotrequiremonitoringofpH.Unlikeotherkits,thereisnoneedtoaddisopropanoltothemeltedagarosepriortoloADIngonthecolumn,savingyouastep.ElutedDNAisreadyforuseinrestrictiondigests,DNAsequencing,ligationandotherenzymaticmanipulations.Monarch?NucleicAcidPurificationKits–DesignedwithsustainABIlityinmind,MonarchkitsusesignificantlylessplasticandresponsIBLy-sourced,recyclablepackaging

      Viewourvideosonprotocols,tips,andrecyclingMonarch.

      MonarchDNACleanupColumnDesign
      Monarch DNA Cleanup Column Design
      Monarchcolumnsaredesignedforperformance
      Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
      Monarchcolumnsaredesignedwithoutafrit,whicheliminatesbufferretentionandtheriskofcarryovercontamination,providingfast,worry-freeDNApurification.

      Advantages:
      • Eluteinaslittleas6μl
      • Preventbufferretentionandsaltcarry-overwithoptimizedcolumndesign
      • Savetimewithfast,user-friendlyprotocols
      • NoneedtomonitorpHoraddisopropanol
      • Buffersandcolumnsavailableseparately
      • Significantlylessplasticusedwhencomparedwithotherkits
      • Responsibly-sourcedandrecyclablepackaging

      Specifications
      DNASampleType:double-strandedDNAfromagarosegels
      BindingCapacity:upto5μg
      DNASizeRange:~50bpto25kb
      TypicalRecovery:DNA(50bpto10kb):70–90%
      DNA(11–23kb):50–70%
      ElutionVolume:≥6μl
      Purity:A260/280>1.8
      ProtocolTime:10minutesofspinandincubationtime
      CompatibleDownstream
      Applications:      		ligation,restrictiondigestion,labelingandotherenzymatic
      manipulations,libraryconstructionandDNAsequencing.

      MonarchDNAGelExtractionKitProtocol?
      Monarch DNA Gel Extraction Kit Protocol
      DNApurifiedfromtheMonarchDNAGelExtractionKitisrecoveredwithsimilarefficiencyandpurityastheleadingsupplier,butismorehighlyconcentrated,facilitatingitsuseindownstreamapplications
      DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications. One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop? readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.
      Onemicrogramaliquotsofa3kbfragmentwereresolvedona1%w/vagarosegel,excised,andprocessedwithdifferentkitsusingmanufacturer-specifiedminimumelutionvolumes.ValuesreportedaretheconcentrationandpuritydatadeterminedbyNanodrop?readings,aswellasrecoverycalculationsbasedontheelutedDNAconcentrationandrecoveredvolume.?
      MonarchDNAGelExtractionKitreproduciblyrecoversDNAoverabroadrangeofmolecularweights.?
      Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.
      Amixtureof7DNAfragmentsrangingfrom10kbdownto0.5kbwaspreparedandone-halfofthemixturewasresolvedona1%gel.EachfragmentwasmanuallyexcisedfromtheagarosegelandprocessedusingtheMonarchDNAGelExtractionKit.Theentireelutionofeachfragmentwasresolvedonanewgelwiththeremainderoftheoriginalmixtureforcomparison.?
      DNApurifiedfromagarosegelsusingtheMonarchDNAGelExtractionKitcanbereproduciblyisolatedandligated.
      DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367 (https://www.neb.com/products/m0367-blunt-ta-ligase-master-mix) ). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232 (https://www.neb.com/products/n3232-1-kb-dna-ladder) ).
      Twomicrogramsofa3kbfragmentwithcompatibleendswasresolvedona1%agarosegel,excised,andpurifiedusingtheMonarchDNAGelExtractionKit.Sampleswereelutedin20μlandafraction(1/4thoftotal)wasligatedusingtheBlunt/TALigaseMasterMix(NEB#M0367).Representativesamplesfrom5replicateswereresolvedonasecond1%agarosegel.Misthe1kbDNALadder(NEB#N3232).

      LearnMoreAboutMonarch
      • NEBMonarch.com
      • Optimizeyourresultswithouruniquecolumndesign
      • EnhanceyourDNApurificationexperience
      • FeelgoodaboutchoosingMonarch
      • ChooseMonarchkitsforpurevalue

      KitComponents

      Thefollowingreagentsaresuppliedwiththisproduct:

      Storeat(°C)Concentration
      Monarch®GelDissolvingBuffer251X
      Monarch®DNAWashBuffer255X
      Monarch®DNAElutionBuffer251X
      Monarch®DNACleanupColumns(5μg)25

      Notes:

      Thekitshouldbestoredatroomtemperature.Alwayskeepbufferbottlestightlyclosedandkeepcolumnssealedintheenclosedzip-lockbag.Forinformationregardingthecompositionofbuffers,pleaseconsulttheSafetyDataSheets.Properlaboratorysafetypracticesshouldbeemployed,includingtheuseoflabcoats,glovesandeyeprotection.
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