Description: 1.2% tryptone, 2.4% yeast extract, 72mM Potassium Phosphate diabasic, 17mM Potassium Phosphate monobasic and glycerol. The 454 gram pouch comes with 300ml of glycerol. The 1 kilogram size comes with 500ml of glycerol.? When the glycerol is added the final Application: For use in propagation bacteriophage lamda.? Use 50.9 grams and 16ml of glycerol per liter to reconstitute media. Shipping Conditions: Ambient. Storage Conditions: Room Temperature.