Goat polyclonal antibody raised against synthetic peptide of AAK1.
Immunogen:
A synthetic peptide corresponding to amino acids at internal region of human AAK1.
Sequence:
C-GSPRTSQQNVYNPSE
Host:
Goat
Theoretical MW (kDa):
104
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:16000)Western Blot (1-3 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.Henderson DM, Conner SD.Mol Biol Cell. 2007 Jul;18(7):2698-706. Epub 2007 May 9.
Applications
Western Blot (Tissue lysate)
AAK1 polyclonal antibody (Cat # PAB17258) (1 ug/mL) staining of human amygdala lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
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