Rat Adipoq (NP_653345) full-length recombinant protein expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
30
Form:
Lyophilized
Purity:
>= 90% by SDS-PAGE
Endotoxin Level:
< 0.1 ng/ug
Activity:
Measured by its ability to inhibit proliferation of mouse myeloid cell line M1 (Yokota, T. et al., Blood 96:1723, 2000). The ED50 for the effect is typically 1-7 ug/mL.
Storage Buffer:
Lyophilized from 25 mM Tris, 0.15 M NaCl, pH 7.4 (50 ug BSA per 1 ug protein)
Storage Instruction:
Store at -20°C on dry atmosphere.After reconstitution with PBS containing at least 0.1% BSA, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.