Mouse Casp1 (P29452) recombinant protein expressed in Escherichia coli. This protein composed of large (20 KDa) and small (10 KDa) subunits. The protein contains about 284 aa without propeptide and fused with HIS tag at N-terminus. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA).
Host:
Escherichia coli
Form:
Lyophilized
Preparation Method:
Escherichia coli expression system
Activity:
>2000 units/mg
Storage Buffer:
No additive
Storage Instruction:
Store lyophilized product at -80°C on dry atmosphere.After reconstitution with PBS, the enzyme should be aliquoted and immediately stored at -80°C.Aliquot to avoid repeated freezing and thawing.Centrifuge the vial prior to opening.
Note:
Reconstitution Instructions:Reconstitute to 1 unit per uL in PBS containing 15% glycerol.Unit Defination:One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.