ProteinaseKisahighlyreactivenonspecificserineproteasethatbelongstothesubtilisinfamilyofproteins.Itcleavesatthecarboxylicacidsideofaliphatic,aromatic,orhydrophobicaminoacids.ProteinaseKiscapableofinactivatingRNasesandDNasesandisusedintheisolationorpreparationofhighmolecularweightnucleicacids.ProteinaseKisalsousefulforhelpingtocharacterizeenzymes,duetoitscleavagespecificity.ThisenzymewasdesignatedproteinaseKbecauseofitsABIlitytohydrolyzekeratin.ProteinaseKisstableinawidevarietyofdetergentsandbuffersaltsandatvarioustemperaturesandpH.TheisoelectricpointofproteinaseKis8.9.
ProteinaseKistypicallyusedataconcentrationof50-100μg/ml.ItisactivewithorwithoutthepresenceofSDS,urea,EDTAorvariousmetalions,buttheactivityofproteinaseKcanbeincreasedbyaddingthedenaturingagentsandthestructureofproteinaseKcanbestabilizedbyadditionofCa2+.ProteinaseKisinactivatedbyheatingto95°Cfor10minutesorusinganinhibitorsuchasPMSF,AEBSForDFP.
ProductSpecifications
ProteinaseK,RNase/DNasefree
MOLECULARBIOLOGYGRADE
MW:28.5kDa
Activity:≥30U/mg
UnitDefinition:Oneunitisdefinedastheamountofenzymethatwillliberate1.0μmoltyrosine(Folin-positiveaminoacid)fromcaseinperminuteat37°C,pH7.5.
Storage/Handling:Storeat-20°C.
Reconstitutein50mMTris-HCl(pH8.0),3mMCaCl2.