當(dāng)前位置 ： Millipore >>> Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL
Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL
  • Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL

Millipore/MABE197 | Anti-phospho-Retinoblastoma (Thr821) Antibody, clone E231, rabbit monoclonal/MABE197/100 µL

價(jià)格: 試用 市場價(jià): 0.00
貨號(hào): MABE197
品牌: Millipore
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumberMABE197
      DescriptionAnti-phospho-Retinoblastoma(Thr821)Antibody,cloneE231,rabbitmonoclonal
      AlternateNames
      • retinoblastomasUSPectibilityprotein
      • retinoblastoma-associatedprotein
      BackgroundInformationRetinoblastomatumorsuppressorprotein(Rb)isassociatedwithDNAbindingactivityandisthoughttoplayasignificantroleincontrollingcellcycleprogressionduringtumorgrowth(1,2).Cellcycle-dependentphosphorylationbycdk’sinhibitsRbbinding,thusallowingcellcycleprogression(3).RbinactivationandcellcycleprogressionlikelyrequiresfirstphosphorylationbycyclinDcdk4/6followedbycyclinE-cdk2phosphorylation(4).
      ProductInformation
      FormatDilutedCultureSupernatant
      Control
      • PhosphataseuntreatedandtreatedJurkatcelllysates
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow
      -20°CmaycauseglycerolcontainingSolutionstobecomefrozenduringstorage.
      Applications
      ApplicationDetectphospho-RetinoblastomawithAnti-phospho-Retinoblastoma(Thr821)Antibody,cloneE231(RabbitMonoclonalAntibody),thathasbeenshowntoworkinWB,ICC&IP.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunoprecipitation
      ApplicationNotesImmunoprecipitationAnalysis:1:20dilutionfromarepresentativelotwasusedinIP.
      ImmunocytochemistryAnalysis:1:100-1:250dilutionfromarepresentativelotdetectedRbinHeLacells.
      BIOLOGicalInformation
      ImmunogenSyntheticphospho-peptidecorrespondingtohumanRbphosphorylatedatThr821.
      EpitopePhosphorylatedThr821
      CloneE231
      HostRabbit
      IsotypeIgG
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      GeneSymbol
      • retinoblastoma1
      • RB
      • p105-Rb
      • pp110
      • pRb
      • osteosarcoma
      • OSRC
      • Rb
      Modifications
      • Phosphorylation
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.ActsasatranscriptionrepressorofE2F1targetgenes.Theunderphosphorylated,activeformofRB1interactswithE2F1andrepressesitstranscriptionactivity,leADIngtocellcyclearrest.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.InhibitstheintrinsickinaseactivityofTAF1.MediatestranscriptionalrepressionbySMARCA4/BRG1byrecruitingahistonedeacetylase(HDAC)complextothec-FOSpromoter.Inrestingneurons,transcriptionofthec-FOSpromoterisinhibitedbyBRG1-dependentrecruitmentofaphospho-RB1-HDAC1repressorcomplex.Uponcalciuminflux,RB1isdephosphorylatedbycalcineurin,whichleadstoreleaseoftherepressorcomplexBysimilarity.Incaseofviralinfections,interactionswithSV40largeTantigen,HPVE7proteinoradenovirusE1AproteininducethedisassemblyofRB1-E2F1complextherebydisruptingRB1"sactivity.
      SUBUNITSTRUCTURE:InteractswithATAD5.InteractswithPRMT2Bysimilarity.ThehypophosphorylatedforminteractswithandsequesterstheE2F1transcriptionfactor.InteractswithheterodimericE2F/DPtranscriptionfactorcomplexescontainingTFDP1andeitherE2F1,E2F3,E2F4orE2F5,orTFDP2andE2F4.TheunphosphorylatedforminteractswithEID1,ARID3B,KDM5A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithNDC80.InteractswithGRIP1andUBR4.InteractswithARID4AandKDM5B.InteractswithE4F1andLIMD1.InteractswithSMARCA4/BRG1ANDHDAC1Bysimilarity.InteractswithadenovirusE1Aprotein,HPVE7proteinandSV40largeTantigen.InteractswithPSMA3andUSP4.Interacts(whenmethylatedatLys-860)withL3MBTL1.
      SUBCELLULARLOCATION:Nucleus.
      TISSUESPECIFICITY:Expressedintheretina.
      DOMAIN:ThePocketdomainbindstothethreonine-phosphorylateddomainC,therebypreventinginteractionwithheterodimericE2F/DPtranscriptionfactorcomplexes.
      PTM:PhosphorylatedinG1,therebyreleasingE2F1whichisthenabletoactivatecellgrowth.DephosphorylatedatthelateMphase.SV40largeTantigen,HPVE7andadenovirusE1Abindtotheunderphosphorylated,activeformofpRb.PhosphorylationatThr-821andThr-826promotesinteractionbetweentheC-terminaldomainCandthePocketdomain,andtherebyinhibitsinteractionswithheterodimericE2F/DPtranscriptionfactorcomplexes.DephosphorylatedatSer-795bycalcineruinuponcalciumstimulation.
      N-terminusismethylatedbyMETTL11A/NTM1Bysimilarity.MonomethylatedatLys-860bySMYD2,promotinginteractionwithL3MBTL1.
      INVOLVEMENTINDISEASE:DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil("cateye")isinvestigated.
      DefectsinRB1areacauseofsusceptibilitytobladdercancer(BLC)[MIM:109800].Amalignancyoriginatingintissuesoftheurinarybladder.Itoftenpresentswithmultipletumorsappearingatdifferenttimesandatdifferentsitesinthebladder.Mostbladdercancersaretransitionalcellcarcinomas.Theybeginincellsthatnormallymakeuptheinnerliningofthebladder.Othertypesofbladdercancerincludesquamouscellcarcinoma(cancerthatbeginsinthin,flatcells)andadenocarcinoma(cancerthatbeginsincellsthatmakeandreleasemucusandotherfluids).Bladdercancerisacomplexdisorderwithbothgeneticandenvironmentalinfluences.
      DefectsinRB1areacauseofosteogenicsarcoma(OSRC)[MIM:259500].
      SEQUENCESIMILARITIES:Belongstotheretinoblastomaprotein(RB)family.
      MolecularWeight~110observed
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知