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Millipore/MAB5414 | Anti-Notch 1 Antibody, extracellular, clone 8G10/MAB5414/100 µL
  • Millipore/MAB5414 | Anti-Notch 1 Antibody, extracellular, clone 8G10/MAB5414/100 µL

Millipore/MAB5414 | Anti-Notch 1 Antibody, extracellular, clone 8G10/MAB5414/100 µL

價格: ￥4308.00 市場價: 7180.00
貨號: MAB5414
品牌: Millipore
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    • Description
      CatalogueNumberMAB5414
      Replaces04-1046
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Notch1Antibody,extracellular,clone8G10
      AlternateNames
      • MotchA
      • mT14
      • p300
      BackgroundInformationNotch1isatype1membraneproteinthatservesasareceptorforthemembraneboundligandsjagged-1and-2anddelta-1toregulatecellfatedeterminationthroughtheimplementationofdifferentiation,proliferationandapoptoticprograms.Uponligandbinding,theNotchintracellulardomain(NICD)isreleasedbyproteolyticprocessingandsubsequentlytranslocatestothenucleuswhereitformsatranscriptionallyactivecomplexwithRBP-Jkappatoactivategenesoftheenhancerofsplitlocus.Notch-1mayalsobeinvolvedincellspecificationand/ordifferentiationandbodysegmentdeterminationinthepostimplantationembryo.Intheadultanimal,Notch-1ishighlyexpressedinthebrain,lung,andthymuswithlowerexpressionlevelsinthespleen,bone-marrow,spinalcord,eyes,mammarygland,liver,intestine,skeletalmuscle,kidneyandheart.
      ProductInformation
      FormatCultureSupernatant
      Control
      • POSITIVECONTROL:brain,lung,thymus.
      PresentationUnpurifiedtissueculturesupernatantfromaperfusionsystem,filteredthrougha0.2micronmembranepriortovialing.Productcontains20%FBSandCiprofloxacinatfinalconcentrationof10μg/mL.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationUseAnti-Notch1Antibody,extracellular,clone8G10(SyrianHamsterMonoclonalAntibody)validatedinFC,WB,IHCtodetectNotch1alsoknownasMotchA,mT14,p300.
      KeyApplications
      • FlowCytometry
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot

      Immunohistochemistryonparaformaldehydefixedfrozentissuesections.

      FlowCytometry

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenAGSTfusionproteincomprisingaminoacids1299-1492ofmouseNotch1
      Epitopeextracellular
      Clone8G10
      HostSyrianHamster
      SpecificityReactswithnaturalandrecombinantNotch1.Recognizesabandmigratingatapproximately300kDaonWesternblotsofratandmouseproteinextracts.Acrossreactivebandmigratingatapproximately230kDaisobservedinsomesamplesandisbelievedtocorrespondtotheprocessedextracellularfragmentofNotch1.
      IsotypeIgG1
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.
      GeneSymbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.MaybeimportantforfolliculardifferentiationandpossIBLycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity).
      SIZE:2556aminoacids;272500Da
      SUBUNIT:HeterodimerofaC-terminalfragmentN(TM)andanN-terminalfragmentN(EC)whichareprobablylinkedbydisulfidebonds.InteractswithDNER,DTX1,DTX2andRBPSUH.AlsointeractswithMAML1,MAML2andMAML3whichactastranscriptionalcoactivatorsforNOTCH1.
      SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).&Notch1intracellulardomain:Nucleus(Bysimilarity).Note=FollowingproteolyticalprocessingNICDistranslocatedtothenucleus(Bysimilarity).
      TISSUESPECIFICITY:Infetaltissuesmostabundantinspleen,brainstemandlung.Alsopresentinmostadulttissueswhereitisfoundmainlyinlymphoidtissues.
      PTM:Synthesizedintheendoplasmicreticulumasaninactiveformwhichisproteolyticallycleavedbyafurin-likeconvertaseinthetrans-Golginetworkbeforeitreachestheplasmamembranetoyieldanactive,ligand-accessibleform.CleavageresultsinaC-terminalfragmentN(TM)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleavedbyTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcallednotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilindependentgamma-secretasetoreleaseanotch-derivedpeptidecontainingtheintracellulardomain(NICD)fromthemembrane(Bysimilarity).&Phosphorylated(Bysimilarity).
      DISEASE:SwissProt:P46531#NOTCH1truncationisassociatedwithT-cellacutelymphoblasticleukemia.&DefectsinNOTCH1areacauseofaorticvalvedisease[MIM:109730].Thedisorderconsistsofanearlydevelopmentaldefectintheaorticvalveandalaterde-repressionofcalciumdepositionthatcausesprogressiveaorticvalvedisease.CalcificationoftheaorticvalveisthethirdleADIngcauseofheartdiseaseinadults.Theincidenceincreaseswithage,anditisoftenassociatedwithabicUSPidaorticvalvepresentin1-2%ofthepopulation.
      SIMILARITY:SwissProt:P46531##BelongstotheNOTCHfamily.&Contains5ANKrepeats.&Contains36EGF-likedomains.&Contains3LNR(Lin/Notch)repeats.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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