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Millipore/MAB5402 | Anti-Dynamin 1 Antibody, neuronal specific, clone D5/MAB5402/100 µg
  • Millipore/MAB5402 | Anti-Dynamin 1 Antibody, neuronal specific, clone D5/MAB5402/100 µg

Millipore/MAB5402 | Anti-Dynamin 1 Antibody, neuronal specific, clone D5/MAB5402/100 µg

價格: ￥3948.00 市場價: 6580.00
貨號: MAB5402
品牌: Millipore
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    • Description
      CatalogueNumberMAB5402
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Dynamin1Antibody,neuronalspecific,cloneD5
      BackgroundInformationDynaminisa100kDaGTPbindingproteinthatwasfirstidentifiedasaproteinabletobindmicrotubules.AmutationintheDrosophilageneshits,encodingadynaminhomologue,preventssynapticvesiclerecyclingandcausestemperature-sensitiveparalysis,

      implicatingdynaminintheformationofcoatedvesicles.Dynaminmaymediatevesicleformationbyassemblingintoaringstructurearoundthenecksofinvaginatedcoatedpitsthatmayeventuallyleadtotheclosureoftheneckandformationofvesicles.Amphiphysin,dynamin,clathrinandAP2formacomplexduringsynapticvesicleendocytosis,whichisregulatedbythephosphorylatedstateofdifferentcomponentsinthiscomplex.To

      date,threegeneshavebeenfoundtoencodedynaminisoforms.Dynamin1isneuronalspecific,Dynamin2isubiquitouslyexpressedandDynamin3localizestotestis,brainandlung.
      ProductInformation
      FormatPurified
      PresentationPurifiedimmunoglobulin.Liquidcontaining0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.

      ImportantNote:Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer"scap.
      Applications
      ApplicationThisAnti-Dynamin1Antibody,neuronalspecific,cloneD5isvalidatedforuseinWB,IHforthedetectionofDynamin1.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblotonhuman,ratandmousebrainhomogenate:Brainswereisolatedrodentsandhomogenizedat2,800rpmwithaPotter-Elvehjemtypehomogenizerafteradding3volof4°CBufferA(10mMHepes,pH7.2,25mMsucrose,and10mMEGTA)containingproteaseinhibitors(1mMphenylmethylsulfonylfluoride,100μg/mlsoybeantrypsininhibitor,10μg/mlleupeptin,10μg/mlp-toluenesulfonyl-L-argininemethylester,and5μg/mlpepstatinA).Thehomogenatewascentrifuged15minat13,000gthen1hat100,000g,andthesupernatantwascollectedascrudesynaptosomalfraction.ThisfractionwassubjectedtoSDS-PAGEandWesternblotanalysis.Detectsabandofapproximately100kDa(97.4kDa).

      Immunofluorescenceonratbraintissuethathasbeenperfusedandfixedwithformaldehyde,7microncutsectionsfrozensections.Hasnotbeentestedonparaffinembeddedtissuesections.

      ImmunoprecipitationonTritonX-100extractsofpost-nuclearsupernatantofratbrainhomogenate:brainsfrommaleSpragueDawleyratswerehomogenizedasdescribedabovein4volof4°CRIPAbuffer(50mMTris,pH8.0,150mMNaCl,1mMEDTA,1%TritonX-100,0.5%deoxycholate,and0.1%SDS)containingproteaseinhibitors.Thehomogenatewascentrifugedasaboveandthesupernatantcollectedastheclarifiedextract.Appropriateantibodies(5μg)wereincubatedwith1-mlfractions(15-25mg)oftheclarifiedextracts,andthenpreswollenproteinGbeads(5mg)wereaddedbeforeasecondincubation.Incubationswere2-12hat4°Cwithrotation.Beadsandimmunecomplexeswerecollectedbycentrifuging3minat7,000gandthenrinsed4×5minwiththestartingbufferbeforebeingsubjectedtoSDS-PAGEandWesternblotanalysis.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenRecombinantHis-taggedfusionprotein,correspondingtoaminoacids1-750ofHumanDynamin1.
      Epitopeneuronalspecific
      CloneD5
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityReactsDynamin1.Microtubuleassociatedprotein
      IsotypeIg
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthedynaminsubfamilyofGTP-bindingproteins.Theencodedproteinpossessesuniquemechanochemicalpropertiesusedtotubulateandsevermembranes,andisinvolvedinclathrin-mediatedendocytosisandothervesiculartraffickingprocesses.Actinandothercytoskeletalproteinsactasbindingpartnersfortheencodedprotein,whichcanalsoself-assembleleADIngtostimulationofGTPaseactivity.Morethansixtyhighlyconservedcopiesofthe3"regionofthisgenearefoundelsewhereinthegenome,particularlyonchromosomesYand15.Alternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeendescribed.
      GeneSymbol
      • DNM1
      • dynamin
      • dynamin1
      • Dynamin-1
      • DNM
      • EC3.6.5.5
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q05193#Microtubule-associatedforce-producingproteininvolvedinproducingmicrotubulebundlesandabletobindandhydrolyzeGTP.Mostprobablyinvolvedinvesiculartraffickingprocesses,inparticularendocytosis.
      SIZE:864aminoacids;97407Da
      SUBUNIT:InteractswithCAV1andSH3GLB1.BindsSH3GL1,SH3GL2andSH3GL3(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm.Cytoplasm,Cytoskeleton.Note=Microtubule-associated.
      SIMILARITY:SwissProt:Q05193##Belongstothedynaminfamily.&Contains1GEDdomain.&Contains1PHdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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