當(dāng)前位置 ： Millipore >>> Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL
Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL
  • Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL

Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): MAB4078
品牌: Millipore
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問(wèn)題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問(wèn)
咨詢顧問(wèn)

全國(guó)免費(fèi)服務(wù)熱線

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見問(wèn)題
    • Description
      CatalogueNumberMAB4078
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-PCNAAntibody,clone131-11912
      AlternateNames
      • ProliferatingCellNuclearAntigen
      • DNAPolymerasedeltaProcessivityFactor
      ProductInformation
      FormatAscites
      PresentationAscitesfluidcontainingnopreservatives
      StorageandShippingInformation
      StorageConditionsMaintainfrozenat-20°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-PCNAAntibody,clone131-11912isamousemonoclonalantibodyfordetectionofPCNAalsoknownasProliferatingCellNuclearAntigen,DNAPolymerasedeltaProcessivityFactor&hasbeenvalidatedinIF,ICC,IHC(P).
      KeyApplications
      • Immunofluorescence
      • Immunocytochemistry
      • Immunohistochemistry(Paraffin)
      ApplicationNotesImmunohistochemistryonformalinfixed,deparaffinizedtissuesections
      Frozentissuesections.
      Smears.
      Cytospins.
      *DonotuseSeruminblockingsteps.

      Alsoseehttp://ajp.amjpathol.org/cgi/content/abstract/141/6/1453forimportantreactivityinformationon19A2inparaffinfixedtissues.

      Finalworkingdilutionsmustbedeterminedbyenduser.

      IMMUNOHISTOCHEMICALPROCEDUREFORMAB4078

      ThefollowinghistochemicalimmunoperoxidaseprotocolhasbeenoptimizedforusewithMAB4078.

      METHODOLOGY:ThismethodisusedwithstandardstreptavidinorABCimmunoperoxidasemethodsusingaDABsubstraterunatroomtemperaturewithparaffinorfrozensections,smearsorcytospins.

      NOTE:Optimalfixationtimeforparaffinprocessedtissuefixedin10%NeutralBufferedFormalinis12-14hours.

      REQUIREDSUPPLEMENTALMATERIAL:

      1.3%HydrogenPeroxide:commerciallyavailable.

      2.PBS

      3.EthylAlcohol:histologygradeorequivalent

      4.Methanol:histologygradeorequivalent

      5.Acetone:histologygradeorequivalent

      6.Xylene(histologygrade)orXyleneSubstitute(Hemo-D)

      7.MountingMedia:Krystalon(Harleco)orequivalent

      8.Gill#1Hematoxylin:Sigma#GHS-1-32orequivalent

      9.GoatAnti-MouseBiotin

      10.StreptavidinHRP(optionalreagent)

      11.3,3"Diaminobenzidinetetrahydrochloride(DAB):Sigma#D-5637orequivalent

      12.ABC:VectorKit#PK4000orequivalent

      REAGENTPREPARATION:

      1.Primaryantibody:PrepareastocksolutionofantibodyinPBS

      2.GoatAnti-MouseBiotin:Approximatedilutionof1:100inPBS(10mg/test)

      3.Streptavidin:5mLstocksolutionand995mLPBS(1/200dilution).Letsit15minutesbeforeuse(yieldasavolumesufficientfor~20slides)ORifusingABCkitfromVectorfollowasinstructed.

      4.DAB:Reconstitute1.5mgofpowderwith100mLofPBS.Activate15minutespriortousebyadding100mL3%H2O2(yieldsavolumesufficientfor~20slides).WARNING:DABisapossIBLecarcinogen.Avoidcontactwithskin.

      5.PBS:Usingagraduatedcylinder,reconstitutewith500mLofdistilledwater.

      6.AlcoholBaths:Alldilutionsarealcoholsmadewithdistilledwater.

      PROCEDURE:

      PARAFFINSECTIONS

      (cutat4-5microns,10%NeutralBufferedFormalinfixed):

      1.Placeslidesin62°Covenfor15minutes.

      2.PlaceslidesinHemo-D,orxylene,for15minutes.

      3.De-paraffinizeslidesthroughbathsof:A)xylene/100%ethanol1:1(orHemo-D/100%ethanol1:1),B)100%ethylalcoholx2,C)95%ethylalcohol,D)90%ethylalcohol,E)80%ethylalcohol,F)70%ethylalcoholx2(2minuteseachbath).

      4.Placeslidesinrunningcooltapwaterfor5minutes.

      5.Shakeoffexcesswater.Placeslidesonincubationracksheldatroomtemperature.CirclesectionswithPAPpen(Newcomer,Inc.)ordiamondpentoisolatesection.

      6.Floodsectionswithprimaryantibodyandincubatefor35-40minutes.

      7.WipeoffexcessPBS.FloodsectionswithGoatAnti-MouseBiotinreagentandincubatefor35-40minutes.

      8.WashwithPBS3-5min

      9.WashagainwithPBS3-5min.

      10.WipeoffexcessPBS.Floodsectionswithstreptavidin-HRPandincubatefor10minutesORfloodsectionswithABCandincubatefor35-40minutes.

      11.WashwithPBS3-5min.

      12.WipeoffexcessPBS.FloodsectionswithDABandincubatefor6-8minutes.

      13.Placeslidesincoolrunningtapwaterfor5minutes.

      14.PlaceslidesinGills#1hematoxylinfor90seconds.

      15.Placeslidesincoolrunningtapwaterfor5minutes.

      16.DehydrateslidesthroughbathsofA)70%ethylalcohol,B)80%ethylalcohol,C)90%ethylalcohol,D)95%ethylalcoholx2,E)100%ethylalcoholx2,F)xylene/100%ethanol1:1(orHemo-D/100%ethanol1:1)x2,G)xyleneorHemo-Dx2(2minuteseachbath.

      17.Mountslidesusingmountingmedia.

      FROZENSECTIONS(cutat8microns):Pullsectionsfromfreezer(orcutfresh),airdryatroomtemperature(orunderafanfor15minutes),fixedin4oCacetonefor4minutes,airdryfor30minutesatroomtemperature(orunderafanfor15minutes),thenfollowaboveprocedurebeginningat#5.

      SMEARS:Methanolfixfor30minutesat4oC,thenfollowaboveprocedurebeginningat#5.

      CYTOSPINS:CelllinesandothercellpopulationspreparedbyresUSPending,ineachwell,~40,000cellsusing500mL1%NormalGoatSerumperwell.Centrifugefor6minutesat1200RPM.Airdryfor30minutesatroomtemperature(orunderafanfor15minutes).Iftumorcellsarebeingscreened,fixinRTacetonefor2-3minutes.Ifperipheralbloodprepsarebeingscreened,fixin100%methanolfor20minutes.Airdrytheslidesfor30minutesatroomtemperature(or15minutesunderafan)andbeginat#5above.

      NOTE:WhenstainingusingthePCNAantibodiesastheprimaryDONOTuseNormalGoatSerumoranyotherserumblockingstep.
      BIOLOGicalInformation
      ImmunogenPurifiedPCNA
      Clone131-11912
      HostMouse
      SpecificitySpecificforPCNAa36kDaproliferationassociatedantigen.
      IsotypeIgM
      SpeciesReactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Fish
      • Vertebrates
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisfoundinthenucleusandisacofactorofDNApolymerasedelta.TheencodedproteinactsasahomotrimerandhelpsincreasetheprocessivityofleADIngstrandsynthesisduringDNAreplication.InresponsetoDNAdamage,thisproteinisubiquitinatedandisinvolvedintheRAD6-dependentDNArepairpathway.Twotranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.Pseudogenesofthisgenehavebeendescribedonchromosome4andontheXchromosome.
      GeneSymbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P12004#ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleadingstrand.
      SIZE:261aminoacids;28769Da
      SUBUNIT:Homotrimer.InteractswithKCTD10(Bysimilarity).Formsacomplexwithactivator1heteropentamerinthepresenceofATP.InteractswithPOLH,POLK,DNMT1,ERCC5/XPG,FEN1,CDC6,APEX2andPOLDIP2.InteractswithEXO1andSHPRH.
      SUBCELLULARLOCATION:Nucleus.
      PTM:Uponmethylmethanesulfonate-inducedDNAdamage,mono-ubiquitinatedbytheUBE2B-RAD18complexonLys-164.Thisinducesnon-canonicalpoly-ubiquitinationonLys-164through"Lys-63"linkageofubiquitinmoietiesbytheE2complexUBE2N-UBE2V2andtheE3ligaseSHPRH,whihisrequiredforDNArepair.
      DISEASE:SwissProt:P12004#Antibodiesarepresentinserafrompatientswithsystemiclupuserythematosus.
      SIMILARITY:SwissProt:P12004##BelongstothePCNAfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問(wèn)題夠可以電話咨詢我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知