Application | ThisAnti-OligodendrocytesAntibody,cloneCE-1isvalidatedforuseinIP,IC,IH,IH(P)forthedetectionofOligodendrocytes. |
KeyApplications | - Immunoprecipitation
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry(Paraffin)
|
ApplicationsNotRecommended | |
ApplicationNotes | Immunocytochemistry
Immunohistochemistryat1:1,000-1:5000
NotsuggestedforuseinWesternblot.
Optimalworkingdilutionsmustbedeterminedbyenduser.
IMMUNOHISTOCHEMISTRYPROTOCOLFORMAB328
Thisantibodyhasbeenusedsuccessfullyon30mm,freefloating,4%paraformaldehydefixedratbraintissue.Allstepsareperformedunderconstantagitation.Suggestedprotocolfollows.
1)3x10minutewashesinTBS(withorwithout0.25%Triton).
2)Incubatefor30minutesinTBSwith3%serum(sameashostfromsecondaryantibody).
3)IncubateprimaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody)(withorwithout0.25%Triton)for2hoursatroomtemperaturefollowedby16hoursat4°C.
4)3x10minutewashesinTBS.
5)IncubatewithsecondaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody).
6)3x10minutewashesinTBS.
7)ABCElite(1:200VectorLabs)inTBS.
8)2x10minutewashesinTBS.
9)1x10minutewashinphosphatebuffer(nosaline).
10)DABreactionwith0.06%NiCladdedforintensification.
11)2x10minutewashesinPBS.
12)1x10minutewashinphosphatebuffer(nosaline). |