當前位置 ： Millipore >>> Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
  • Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

價格: ￥3072.00 市場價: 5120.00
貨號: MAB305
品牌: Millipore
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價
  • 品質(zhì)甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumberMAB305
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CholineAcetyltransferaseAntibody,clone1E6
      AlternateNames
      • ChAT
      • CholineAcetylase
      • CHOACTase
      ProductInformation
      FormatAscites
      Control
      • Braintissue
      PresentationAscitesfluidcontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationDetectCholineAcetyltransferaseusingthisAnti-CholineAcetyltransferaseAntibody,clone1E6validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:100-1:250.Seeimmunohistochmistryprocedurebelow.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      IMMUNOHISTOCHEMISTRYPROCEDURE(PAPTECHNIQUE)FORMAB305,MONOCLONALANTIBODYTOCHOLINEACETYLTRANSFERASE

      I)Perfusion&SectioningProcedure

      1.Perfusethroughtheheartwithafixativesolutioncontaining4%paraformaldehydein0.12Mphosphatebuffer(pH7.3)forlightmicroscopy(LM),andadditionally,0.1%gluteraldehydeand.002%CaCl2forelectronmicroscopy(EM).

      2.Removebrainandpostfix2-18hoursat4°Cin4%paraformaldehydein0.12Mphosphatebuffer.

      3.Afterbrainisblockedforsectioning,washinseveralchangesofbufferfor2-3hours.

      4.SpecimensforEMaresectionedonaVibratome(50μm)andrinsedinbuffer,thoseforLMshouldbecryoprotectedin30%sucroseinbuffer.

      5.Afterfreezingwithdryice,30-40μmthicksectionsofLMspecimensarecutonacryostat.

      6.Sectionsarerinsed,andthenstoredinphosphatebuffercontaining0.1%sodiumazide.

      II)StainingProcedure

      Tissueisprocessedasfreely-floatingsectionsincontinuouslyagitatedsolutions.Allincubationsareperformedatroomtemperatureunlessotherwisestated.

      1.a.ForlocalizingChAT-positivesomataanddendrites:

      Sectionsarewashedin0.1MTris-bufferedsaline(TBS;containing1.4%NaCl,pH7.3)only.Nodetergentorenzymepretreatmentisused.

      b.ForlocalizingChAT-positiveterminal-likestructures:

      IncubatesectionsinTBS(pH8.1)for5minutesat37°C.TransfersectionstoTBS(pH8.1)containingpronase(1.2μg/mL)for11/2-2minutesat37°C,followedbyseveralicecoldbufferwashesforatotalof5minutes.Theconcentrationofpronaseandincubationtimeofthedigestionshouldbeevaluatedforeachregionexamined.

      c.ForlocalizingChATimmunoreactivityandsubsequentlycounterstainingthesections:

      IncubationinTBScontaining0.1%-0.8%TritonX-100for15minutesmayincreasethetissuepenetrationoftheImmunoReagents,butitalsoraisesthebackgroundstaining.

      2.Incubatesectionsinnormalgoatserum(3-5%)foronehour.Theworkingsolutionsofallantiserashouldalsocontainsimilarlydilutednormalgoatserum.

      3.Incubateinanti-ChATmonoclonalantibodysolution(Suggestedworkingdilution1:250,finalworkingdilutionmustbedeterminedbyenduser)for2hoursatroomtemperatureandthenforanadditional6-18hoursat4°C.

      4.Incubatewithsecondantibody(i.e.Goatanti-MouseIgG,Cat.No.:AP124,dilution1:50-100)for1-2hours.

      5.IncubatewithdilutedPAPcomplex(i.e.MousePAP,CatNo.:PAP14,conc.25-50μg/mL)foronehour.

      6.Afterrinsinginbuffer,thesecondantibodyandPAPstepsarerepeatedfor40minutesto1houreachinordertoamplifystainingintensity,particularlyofsmallChAT-containingstructures.

      7.Reactfor15minuteswith0.06%3,3"-diaminobenzidine×4HCl(DAB;dilutedinphosphatebufferedsaline,pH7.3)and0.006%H2O2.

      8.SpecimensforroutineLMarepostfixedfor1minutesin0.005%OsO4(osmiumtetraoxide),andthenmounted,dehydratedandcoverslipped.SelectedregionsblockedforEMarepostfixedin2%OsO4for1hour,enblocstainedwithuranylacetate,andflat-embeddedinEpon-Aralditeresin.
      BIOLOGicalInformation
      ImmunogenCholineacetyltransferasepurifiedfromratbrain.
      Clone1E6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizescholinergicneuronsinthebrainandspinalcord(CNS).
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Monkey
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryCholinergicsystemsareimplicatedinnumerousneurologicfunctions.AlterationinsomecholinergicneuronsmayaccountforthedisturbancesofAlzheimerdisease.Theproteinencodedbythisgenesynthesizestheneurotransmitteracetylcholine.Alternativesplicevariantshavebeenfoundthatcontainalternative5"untranslatedexons.Threeofthefourdescribedsplicevariantsencodeidentical69kDaproteinswhileonevariantencodesboththe69kDaandalarger82kDaprotein.
      GeneSymbol
      • CHAT
      • ChAT
      • CMS1A2
      • CHOACTase
      • CMS1A
      • EC2.3.1.6
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P28329#CatalyzesthereversIBLesynthesisofacetylcholine(ACh)fromacetylCoAandcholineatcholinergicsynapses.
      SIZE:748aminoacids;82568Da
      DISEASE:SwissProt:P28329#DefectsinCHATarethecauseoffamilialinfantilemyastheniagravis2(FIMG2)[MIM:254210,254200];alsoknownasCMS-EA.FIMG2patientshavemyasthenicsymptomssincebirthorearlyinfancy,negativetestsforanti-AChRantibodies,andabruptepisodiccriseswithincreasedweakness,bulbarparalysis,andapneaprecipitatedbyundueexertion,fever,orexcitement.Inheritanceisautosomalrecessive.
      SIMILARITY:SwissProt:P28329##Belongstothecarnitine/cholineacetyltransferasefamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態(tài)度，由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關(guān)，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時免費服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯了怎么辦？
    A：同個訂單購買多個商品可能會分為一個以上包裹發(fā)出，可能不會同時送達，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個月內(nèi)（以快遞公司顯示簽收時間為準），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內(nèi)申請退換貨；特殊商品以合同條款為準。

何為188

極簡而嚴謹，我們僅銷售188款生物醫(yī)學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號

獲取最新優(yōu)惠活動通知