BackgroundInformation | Antibodiestoneuronalproteinshavebecomecriticaltoolsforidentifyingneuronsanddiscerningmorphologicalcharacteristicsincultureandcomplextissue.WhilethelabelingfromclassichistologicaltechniquessuchasGolgistainingandmodernmolecularapproachessuchasGFPconstructsyieldexcellentcytoarchitecturaldetail,theseapproachesaretechnicallychallengingandimpracticalformanyneuroscienceresearchneeds.Neuron-specificantibodiesareconvenientprecisiontoolsusefulinrevealingcytoarchitecture,butarelimitedtotheproteintargetdistributionwithintheneuron,whichmaydiffergreatlyfromnucleustosomatodendriteandaxon.ToachieveascompleteamorphologicalstainingaspossIBLeacrossallpartsofneurons,Milliporehasdevelopedamonoclonalantibodyblendthatreactsagainstkeysomatic,nuclear,dendritic,andaxonalproteinsdistributedacrossthepan-neuronalarchitecturethatcanthenbedetectedbyasinglesecondaryantibody.Thisantibodycocktailhasbeenvalidatedindiversefixations,cellcultureandimmunohistochemistryprotocols,includingarchivalbraintissue,givingresearchersaconvienentandspecificqualitativeandquantitativetoolforstudyingneuronalmorphology. |