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Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL
  • Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL

Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL

價(jià)格: ¥3204.00 市場價(jià): 5340.00

貨號: MAB2030
品牌: Millipore
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    • Description
      CatalogueNumberMAB2030
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Chondroitin4SulfateAntibody,cloneBE-123
      ProductInformation
      FormatAscites
      PresentationAscitesfluidcontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-Chondroitin4SulfateAntibody,cloneBE-123detectslevelofChondroitin4Sulfate&hasbeenpublished&validatedforuseinELISA,RIA&WB.
      KeyApplications
      • ELISA
      • RADIoimmunoassay
      • WesternBlotting
      ApplicationNotesELISAat1:400-1:3,200.

      RIAat1:50-1:6,400.

      Immunohistochemistry:Thisantibodyonlyreactswithdigestedmaterial.FixedtissuemustbedigestedwithchondroitinaseABC(Sigma).
      TheReference23757342shouldbeindicatedforIHC.(Reine,TM.,etal.,PubMedID:23757342)

      WesternBlot:1:1000-1:4000usingECLdetectionmethod.ChondroitinaseABCtreatmentoflysatesampleishelpful.
      Inanyalkalibuffer,e.g.Trisoracetate(50mMor100mM)pH7.4-7.6.MakeastocksolutionbydilutingonevialofSigmaChondroitinaseA,B,C,(5units/vial)in100μL.Foruse,dilutestock10-foldintothebufferofchoice.Useataratioof50-100mgofproteoglycanper5unitsofenzyme.Onfixedcells,diluteinTrisacetateandonnon-fixedcellsydilute10-25foldinDMEM.
      Poole,C.A.,etal.,J.Histochem.Cytochem.1991.39:1175-1187.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenMouseproteoglycan.
      CloneBE-123
      HostMouse
      SpecificityReactswithbothmouse(fetalandadult)andhuman(fetalandadult)chondroitinaseABCdigestedcartilageproteoglycans.Chondroitin-4sulfatedisaccharidessuccessfullyinhibitthebindingofmonoclonalantibodytoproteoglycans.NoreactionwithnativeproteoglycansorwithproteoglycansdigestedwitheitherchondroitinaseACortesticularhyaluronidase.
      IsotypeIgG1κ
      SpeciesReactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Rat
      • Sheep
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryAhumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreadingonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells.
      GeneSymbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.
      SIZE:2322aminoacids;250497Da
      SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16.
      SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).
      TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells.
      PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity).
      SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains.
      MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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