Specificity | Humanlatestageinflammatorymacrophages.ThemonoclonalissuitableforuseonBAL(bronchiallavagefluids)andotherlavages.TheantigenrecognizedbyMAB1569isan86kDaprotein(unreducedconditions),probablyaglycoproteinonthecellsurfaceandwithinthecytoplasmofmaturemacrophages.Itisstabletoformaldehydefixationandparaffinembedding.Enzymedigestionisrecommended.Theantibodyhasalsobeenreportedtorecognizemicrogliastemcells(XInternationalCongressofNeuropathology,Stockholm,le11September,1986.PosterA-701).
Antigendistribution:absentfromfreshlyisolatedmonocytesandotherbloodcells;presenton40-50%ofhumanmonocytesafter6-7daysinculture,alsopositiveonsomemelanomaandcarcinomalines.Intissuesections,cloneidentifiesKupffercells,histiocytes(skin),macrophagesofthethymus,integerminalcentersoflymphnodesandspleen,inmammacarcinoma,melanoma,osteocarcinomaandgastriccancer;excema,sarcoidosis,BCGgranuloma;synovialliningcells,tuberculoidleprosy,howevernoexpressioninlepramatousleprosy.
Speciesreactivityisseeninhuman,rhesusmonkey,andpig,otherspeciesnottested. |