當(dāng)前位置 ： NEB >>> NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions
NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions
  • NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions

NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions

價(jià)格: ￥4320.00 市場(chǎng)價(jià): 7200.00
貨號(hào): M0541S-250reactions
品牌: NEB
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國(guó)免費(fèi)服務(wù)熱線

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見問題

    • Description:







      ?
      TheNEBNextHigh-Fidelity2XPCRMasterMixisspecificallyoptimizedfortherobust,high-fidelityamplificationofnext-generationsequencing(NGS)libraries,regardlessofGCcontent.Thepolymerasecomponentofthemastermix,Q5??High-FidelityDNAPolymerase,isanovelThermostableDNApolymerasethatpossesses3′→5′exonucleaseactivity,andisfusedtoaprocessivity-enhancingSso7ddomain.Q5High-FidelityDNAPolymerasealsohasanultra-lowerrorrate(>100-foldlowerthanthatofTaqDNAPolymeraseand6-foldlowerthanthatofPyrococcusfuriosus(Pfu)DNAPolymerase).Thebuffercomponentofthemastermixhasbeenoptimizedforrobustamplification,evenwithGC-richamplicons.

      Figure1:ComparativeAnalysisofDifferentDNAPolymeraseswithKnownLowCoverageRegions.
      Figure 1: Comparative Analysis of Different DNA Polymerases with Known Low Coverage Regions.
      IndexedlibrarieswerepreparedfromhumanIMR90DNAandsplitintoindividualsamplesforlibraryamplification.Amplificationwasperformedusing8cyclesofPCRwithPhusionHigh-FidelityDNAPolymerase,KAPAHiFi?HotStartReadyMixorNEBNext?High-Fidelity2XPCRMasterMix.LibrariesweresequencedonanIlluminaHiSeq?2000.Tocorrectforslightdifferencesinthenumberofalignedreadsfromeachlibrary,180millionreadswererandomlyextractedfromeachdataset,representinganaveragecoverageof~6X.Thenumberofreadsoverlappingdistinctlowcoverageregionsofthehumangenome(Airdet.al.GenomeBIOLOGy,2011)areshownforeachlibrary.TheNEBNextHigh-Fidelity2XPCRMasterMixgivesthemostoptimalperformanceofthethreeenzymes/mastermixestested.
      Figure2:FidelityComparisonsofDifferentDNAPolymerases.
      Figure 2: Fidelity Comparisons of Different DNA Polymerases.
      Fidelitymeasurementsof?Taq?DNAPolymerase(inStandard?Taq?Buffer),KAPAHiFiHotStartReadyMixandNEBNextHigh-Fidelity2XPCRMasterMixweremeasuredside-by-sideinaPCR-basedmutationscreeningassayusingalacZmethodmodifiedfromKermekchievetal.,2003.Values(n≥2)areexpressedrelativetoKAPAHiFiHotStartReadyMix.TheQ5High-FidelityPolymeraseintheNEBNextHigh-Fidelity2XPCRMasterMixhasaleveloffidelity>10XhigherthanthatofthepolymeraseintheKAPAHiFiHotStartReadyMix.
      Figure3:ComparativeAnalysisofDifferentDNAPolymeraseswithGenomesofVarying%GC.
      Figure 3: Comparative Analysis of Different DNA Polymerases with Genomes of Varying % GC.
      LibrariesofH.influenza,R.palustrisorhumangenomicDNAwereamplifiedusingNEBNextHigh-Fidelity2XPCRMasterMix,PhusionHigh-FidelityPCRMasterMixwithHFBufferorKAPAHiFiHotStartPCRReadyMix,andsequencedonanIllumina?HiSeq2000.GCcoverageplotsweregenerated,with%GCcontentof100bpwindowsontheXaxis.Normalizedcoverageisindicatedbythebluecircles,windowsatGC%indicatedbytheredlines,andbasequalityatGC%indicatedbythegreenline.NEBNextHigh-Fidelity2XPCRMasterMixprovidessubstantiallyreducedbiasonallthreegenomicDNAsamples.
      Figure4:ComparativeAnalysisofDifferentDNAPolymerasesatVaryingGC%s.
      Figure 4: Comparative Analysis of Different DNA Polymerases at Varying GC %s.
      AmplifiedlibrariesofhumangenomicDNAweregeneratedusingindexprimersandNEBNextHigh-Fidelity2XPCRMasterMix,Phusion?High-FidelityPCRMasterMixwithHFBufferorKAPAHiFiHotStartReadyMix,andsequencedonanIllumina?HiSeq?2000.AnequalnumberofreadsfromeachdatasetwererandomlyselectedandthepercentageofreadswasplottedagainstGCcontent.NEBNextHigh-Fidelity2XPCRMasterMixandKAPAHiFHotStartReadyMixalignedcloselytotheexpectedreadfrequencies(shadedgrey),whilePhusiondidnot.

      ProductSource

      AnE.colistrainthatcarriestheQ5High-FidelityDNAPolymerasegene.

      ReactionVolumeDefinition

      NEBNextHigh-Fidelity2XPCRMasterMix,DNAtemplateand0.5μMto1.25μMprimers(dependingonsampleinput)inatotalreactionvolumeof50μl.

      Notes:

      Toensureoptimalperformance,themastermixshouldbethawedandresUSPendedpriortouse.StABIlitytestingusingupto20freeze/thawcycleshasshownnonegativeeffectonmastermixperformance.TheNEBNextHigh-Fidelity2XPCRMasterMixmaybeliquidat-20°C.
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知