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NEB/Phusion? Hot Start Flex 2X Master Mix/500 reactions (50 μl)/M0536L

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貨號(hào): M0536L

品牌: NEB

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- High Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error-rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format. The addition of an aptamer-based inhibitor allows room temperature reaction setup. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg⁺⁺, requiring only the addition of primers and DNA template for robust amplification. Reactions can also be optimized using the provided DMSO solution.
  Product Source
  An E. coli strain that carries the Phusion DNA Polymerase gene.
  This product is related to the following categories:
  Phusion® High-Fidelity DNA Polymerases Products,
  Master Mixes Products
  This product can be used in the following applications:
  Hot Start PCR,
  Long Range PCR,
  Fast PCR,
  High-Fidelity PCR,
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  PCR
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