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NEB/Phusion? High-Fidelity PCR Master Mix with HF Buffer/100 reactions (50 μl)/M0531S

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貨號(hào): M0531S

品牌: NEB

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- High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
  Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl₂. All that is required is the addition of template, primers and water.
  Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
  A 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacturer"s recommended conditions. Extension times are indicated (in minutes). Ladder L is a 1 kb DNA Ladder.
  Product Source
  An E. coli strain that carries the Phusion DNA Polymerase gene.
  This product is related to the following categories:
  Phusion® High-Fidelity DNA Polymerases Products,
  Master Mixes Products
  This product can be used in the following applications:
  Long Range PCR,
  Fast PCR,
  High-Fidelity PCR,
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  PCR
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