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NEB/Q5? Hot Start High-Fidelity DNA Polymerase/500 units/M0493L

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貨號(hào): M0493L

品牌: NEB

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- Q5^® Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg⁺⁺ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB T_m Calculator is highly recommended.
  Amplification of a variety of human genomic amplicons fromlow to high GC content using Q5 Hot Start High-Fidelity DNA Polymerase. Allreactions were set up at room temperature, conducted using 30 cycles ofamplification and visualized by microfluidic LabChip^®analysis.
  Product Source
  An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
  This product is related to the following categories:
  Q5® High-Fidelity DNA Polymerases Products
  This product can be used in the following applications:
  Hot Start PCR,
  Long Range PCR,
  Genome Editing Applications,
  Fast PCR,
  High-Fidelity PCR,
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  DNA Amplification, PCR & qPCR,
  PCR,
  Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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