Q5® High-Fidelity DNA Polymeraseis a high-fidelity, thermostable DNA polymerasewith 3´→ 5´ exonuclease activity, fused to aprocessivity-enhancing Sso7d domain to supportrobust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-FidelityDNA Polymerase is ideal for cloning and canbe used for long or difficult amplicons. Q5High-Fidelity DNA Polymerase is supplied withan optimized buffer system that allows robustamplification regardless of GC content. The 5XQ5 Reaction Buffer contains 2 mM Mg++ at final(1X) reaction concentrations and is recommendedfor most routine applications. For GC-rich targets(≥ 65% GC), amplification can be improved bythe addition of the 5X Q5 High GC Enhancer. Q5High-Fidelity DNA Polymerase is unlike typical,lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculatoris highly recommended.
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
This product is related to the following categories:
Q5® High-Fidelity DNA Polymerases Products
This product can be used in the following applications:
Gibson Assembly®,
Long Range PCR,
Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
Specialty PCR,
Routine PCR,
DNA Amplification, PCR & qPCR,
PCR,
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