Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Vent DNA Polymerase (1). Thisis the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase (2,3).
Product Source
AnE. colistrain that carries the Vent (D141A / E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase fromThermococcus litoralis(4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
This product is related to the following categories:
Other PCR Polymerases
This product can be used in the following applications: