常見問題解答:◆我如何為LIVE/DEAD細(xì)胞活力實(shí)驗(yàn)準(zhǔn)備死細(xì)胞對(duì)照?有兩種簡單的方法。一種是通過60°C放置20分鐘熱滅活細(xì)胞。第二種是將細(xì)胞放入70%乙醇。乙醇固定的細(xì)胞可以在冰箱中無限期儲(chǔ)存直到使用,可達(dá)好幾年。步驟:a.離心細(xì)胞、使細(xì)胞沉淀并去除上清b.固定細(xì)胞:在15mL含有細(xì)胞團(tuán)的試管中加入10 mL70%冰乙醇,先開始逐滴加入后震蕩,混合均勻。c.在冰箱中儲(chǔ)存直到使用。d.快使用時(shí),水洗兩次且在選擇的緩沖液中重懸。◆用該試劑盒染色后,可以進(jìn)行后續(xù)固定步驟嗎?染色后不可以進(jìn)行固定步驟。PI作為膜非透性染料,固定后將轉(zhuǎn)移到所有細(xì)胞,不再維持死細(xì)胞特異性染色。
使用本產(chǎn)品的文獻(xiàn):1.Scutellarin potentiates vancomycin against lethal pneumonia caused by methicillin-resistant Staphylococcus aureus through dual inhibition of sortase A and caseinolytic peptidase PAuthor links open overlay panelXingyeWang,LinWei,LiWang,XiaoyuChen,XiangriKong,YanheLuan,JiyuGuan,XueruiGuo,YanShi,TiedongWang,BingmeiWang,WuSong, YichengZhaoBiochemical Pharmacology
參考文獻(xiàn)1.Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki應(yīng)用方向:死活細(xì)胞染色、細(xì)胞計(jì)數(shù)2.Connexin43 hemichannels contribute to cadmium-induced oxidative stress and cell injury應(yīng)用方向:死活細(xì)胞染色、細(xì)胞活力3.An improved fluorescence assay for the determination of lymphocyte-mediated cytotoxicity using flow cytometry應(yīng)用方向:細(xì)胞流式4.Visualization of keratocytes in the human cornea with fluorescence microscopy應(yīng)用方向:組織切片染色