Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins.This resin consists of magnetic agarose derivatized withNitrilotriacetic (NTA) and provides good properties working in native ordenaturing conditions. Magneticagarose beads provide a convenient and quick method for purificationwithout the need for pipetting or centrifugation. Washing, binding andelution steps will require a magnetic device.
NTA cross-linked resins consist of nitrilotriacetic acid groupsligated by stable ether linkages via a spacer arm. NTA is a tetravalentchelating agent whichprovides a higher specificity and lower ion leaching than IDA linkedresins. NTA resins have also been shown to be more robust in the presence ofhigher concentrations of EDTA, but may require a higher imidazoleconcentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.
Nickel NTA Magnetic Beads are supplied as a 5% suspension of magnetic beads in 20% ethanol (each 1 ml of suspension contains 50 μl of magnetic beads).
Loading Capacity (μmol me2+/ml gel): >20 μmol/ ml gel
Binding Capacity: >75 mg His-tagged protein/ ml gel
Storage/Handling: Store at 4°C. Do not freeze.
Note: GoldBio recommends using Nickel NTA resins in the presence of elevated levels of reducing agents in order to ensure optimal purification of your target protein. Nickel IDA resins may show some discoloration in reaction with low levels of reducing agents but are still functional at low levels of reducing agents (≤5mM DTT).
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