Components | - Positive/NegativeControlSlides,Naturalbox
- Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells.
- BlockingBuffer,Whitecap
- H2O2/UreaTablets,Ambervial
- ProteinaseK,Pinkcap
- DABTablets,Ambervial
- TdTEnzyme,Yellowcap
- Br-Dutp,Violetcap
- 200XStreptavidin-HRPConjugate,Blackcap
- 5XReactionBuffer,Greencap
- Biotin-labeledBrdUmAb,Orangecap
- MethylGreensolution,Naturalcap,
- TheAPO-BRDU-IHC?Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival.
- Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.
- Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
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