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Millipore/AB9740 | Anti-14-3-3 Antibody, phospho Serine185/AB9740/100 µL
  • Millipore/AB9740 | Anti-14-3-3 Antibody, phospho Serine185/AB9740/100 µL

Millipore/AB9740 | Anti-14-3-3 Antibody, phospho Serine185/AB9740/100 µL

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貨號: AB9740
品牌: Millipore
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    • Description
      CatalogueNumberAB9740
      Replaces04-1089
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-14-3-3Antibody,phosphoSerine185
      BackgroundInformationMammalsexpresssevendistinct14-3-3isoforms(gamma,epsilon,beta,zeta,sigma,theta,tau)thatformmultiplehomo-andhetero-dimmers,thusrendering14-3-3asakeymultifunctionalregulatorymolecule.14-3-3isomershavebeenimplicatedinseveralneurologicaldisorders:forexampleisoformsgamma,epsilonandbetaareexpressedinthecerebrospinalfluidofCreutzfeldt-Jakobpatients,butnotpatientssufferingfromotherneurologicalconditionssuchasAlzheimer"s.

      The14-3-3phosphoSerine185antibodyisoneofapanelofantibodiesthatweregeneratedagainst14-3-3isoformsandhavedemonstratedtheirpotentialforuseasanELISA-baseddiagnostictool.TheseantibodieswillallowtheresearchtoinvestigatetheroleofthisproteininkeycellularprocessesandneurologicaldisorderssuchasCreutzfeldt-Jakob,Alzheimer"sandParkinson"sDisease.
      ProductInformation
      FormatAffinityPurified
      PresentationLiquidin10mMTris-HClbuffer,pH7.5with150mMNaCl,100ug/mLBSA,50%glyceroland0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetect14-3-3usingthisAnti-14-3-3Antibody,phosphoSerine185validatedforuseinWB.
      KeyApplications
      • WesternBlotting
      ApplicationNotesWesternblot:1:150-1:300usingECL.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticphosphopeptide.
      HostSheep
      Specificity14-3-3phosphoSerine185.ByWesternblottheantibodyrecognizesthe~30kDaprotein.Specificforthephosphorylatedformofbetaandzeta14-3-3thatarefoundendogenouslyphosphorylatedonlyinbraintissue.
      SpeciesReactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneproductbelongstothe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.Thishighlyconservedproteinfamilyisfoundinbothplantsandmammals,andthisproteinis100%identicaltothemouseortholog.ItinteractswithCDC25phosphatases,RAF1andIRS1proteins,suggestingitsroleindiversebiochemicalactivitiesrelatedtosignaltransduction,suchascelldivisionandregulationofinsulinsensitivity.Ithasalsobeenimplicatedinthepathogenesisofsmallcelllungcancer.
      GeneSymbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      Modifications
      • Phosphorylation
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62258#Adapterproteinimplicatedintheregulationofalargespectrumofbothgeneralandspecializedsignalingpathway.Bindstoalargenumberofpartners,usuallybyrecognitionofaphosphoserineorphosphothreoninemotif.Bindinggenerallyresultsinthemodulationoftheactivityofthebindingpartner.
      SIZE:255aminoacids;29174Da
      SUBUNIT:Homodimer.InteractswithNDEL1(Bysimilarity).InteractswithHCVcoreprotein.
      SUBCELLULARLOCATION:Cytoplasm(Bysimilarity).Melanosome.Note=IdentifiedbymassspectrometryinmelanosomefractionsfromstageItostageIV.
      SIMILARITY:SwissProt:P62258##Belongstothe14-3-3family.
      MolecularWeight30kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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