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Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg
  • Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg

Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg

價格: ￥4560.00 市場價: 7600.00
貨號: AB5688
品牌: Millipore
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    • Description
      CatalogueNumberAB5688
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-NeuroD2Antibody
      AlternateNames
      • NDRF
      • NeuroD-relatedFactor
      • NeurogenicDifferentiationFactor2
      • Ndr2
      • BrainbHLHProteinKW8
      BackgroundInformationNeurogenicdifferentiation2,alsoknownasNEUROD2,isahumangene.ThisgeneencodesamemberoftheneuroDfamilyofneurogenicbasichelix-loop-helix(bHLH)proteins.Expressionofthisgenecaninducetranscriptionfromneuron-specificpromoters,suchastheGAP-43promoter,whichcontainaspecificDNAsequenceknownasanE-box.Theproductofthehumangenecaninduceneurogenicdifferentiationinnon-neuronalcellsinXenopusembryos,andisthoughttoplayaroleinthedeterminationandmaintenanceofneuronalcellfates.DiseasesassociatedwithNEUROD2aremotordeficits,ataxia,andseizures.NEUROD2expressionisobservedmainlyinendovascularinvasivecells.
      ProductInformation
      FormatAffinityPurified
      Control
      • Cerebellumtissue
      PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

      Theantibodymustbe"rehydrated"priortouse:
      Torehydrate/reconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammonium
      sulfate/PBSsolution:10mLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

      ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50mgofprecipitatedantibody,theamountofbufferneededwouldbe50mL.

      Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

      Therehydratedantibodycanbestoredat:
      2-8°CTherehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.

      -20°CForstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

      -70°CForfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-NeuroD2AntibodydetectslevelofNeuroD2&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:1:200-1:1,000.
      Immunocytochemistry:1:500onPC12cells
      Westernblot:1:200-1:1,000usingECL.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BiologicalInformation
      ImmunogenSyntheticpeptide,aminoacids23-39ofhumanNeuroD2.
      HostRabbit
      SpecificityRecognizesNeuroD2(NRDL,KW80),aHelix-loop-helixclassoftranscriptionfactor.
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberoftheneuroDfamilyofneurogenicbasichelix-loop-helix(bHLH)proteins.Expressionofthisgenecaninducetranscriptionfromneuron-specificpromoters,suchastheGAP-43promoter,whichcontainaspecificDNAsequenceknownasanE-box.Theproductofthehumangenecaninduceneurogenicdifferentiationinnon-neuronalcellsinXenopusembryos,andisthoughttoplayaroleinthedeterminationandmaintenanceofneuronalcellfates.
      GeneSymbol
      • NEUROD2
      • NDRF
      • NeuroD2
      • MGC26304
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q15784#Appearstomediateneuronaldifferentiation.
      SIZE:382aminoacids;41361Da
      SUBUNIT:EfficientDNAbindingrequiresdimerizationwithanotherbHLHprotein.
      SUBCELLULARLOCATION:Nucleus(Probable).
      SIMILARITY:SwissProt:Q15784##Contains1basichelix-loop-helix(bHLH)domain.
      MolecularWeight41kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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