The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Thr275.
Immunogen Range
241-290
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
MAP2K7 (Phospho-Thr275) Antibody detects endogenous levels of MAP2K7 only when phosphorylated at Thr275.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K7
Database Links
Gene ID: 5609 Swiss-Prot #: O14733
OMIM #: 603014 Unigene #: Hs.531754
Target Modification
Phospho
Modification Sites
H:T275 M:T291 R:T275
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MAP2K7 (Phospho-Thr275) Antibody. The lane on the right is blocked with the phospho peptide.