The antiserum was produced against synthesized peptide derived from human SHIP1 around the phosphorylation site of Tyr1021.
Immunogen Range
987-1036
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
SHIP1 (Phospho-Tyr1021) Antibody detects endogenous levels of SHIP1 only when phosphorylated at Tyr1021.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
INPP5D
Database Links
Gene ID: 3635 Swiss-Prot #: Q92835
OMIM #: 601582 Unigene #: Hs.262886/Hs.601911
Target Modification
Phospho
Modification Sites
H:Y1021 M:Y1020 R:Y1020
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with TNF 200NG/ML 30', using SHIP1 (Phospho-Tyr1021) Antibody. The lane on the right is blocked with the phospho peptide.