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Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/
  • Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

價格: ¥4440.00 市場價: 7400.00

貨號: 506123
品牌: Millipore
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    • Description
      OverviewRecognizesthe~38kDap38MAPKprotein.
    • AntibodyTargetGeneSymbol:MAPK14
    • TargetSynonym:CRK1,CSBP,CSBP1,CSBP2,CSPB1,EXIP,Hog,MAPKp38,MGC102436,MGC105413,MXI2,P38,P38KINASE,P38MapKinase,p38Mapkalpha,P38-ALPHA,p38-RK,p38/Hog1,p38/Mpk2,P38/RK,p38a,p38Hog,p38MAPK,PRKM14,PRKM15,RK,SAPK2A
    • EntrezGeneName:mitogen-activatedproteinkinase14
    • HuEntrezID:1432
    • MuEntrezID:26416
    • RatEntrezID:81649
    • CatalogueNumber506123
      BrandFamilyCalbiochem®
      ApplicationData
      Detectionofp38MAPkinasebyimmunoblotting.Samples:C6cells(lanes1and2)treated(lane1)oruntreated(lane2)withanisomycinandNIH/3T3cells(lanes3and4)treated(lane3)oruntreated(lane4)withUV.Primaryantibody:Anti-p38MAPKinase(341-360)RabbitpAb(Cat.No.506123)(1:1000).Detection:chemiluminescence.
      ProductInformation
      FormLiquid
      FormulationIn150mMNaCl,10mMHEPES,50%glycerol,0.01%BSA,pH7.5.
      PreservativeNone
      StorageandShippingInformation
      ShipCodeBlueIceOnly
      ToxicityStandardHandling
      Storage-20°C
      Avoidfreeze/thawAvoidfreeze/thaw
      DonotfreezeOktofreeze
      SpecialInstructionsFollowinginitialthaw,aliquotandfreeze(-20°C).
      Applications
      KeyApplicationsFlowCytometry
      Immunoblotting(WesternBlotting)
      ParaffinSections
      ApplicationNotesFlowCytometry(1:25)
      Immunoblotting(1:1000)
      ParaffinSections(1:50,heatpretreatmentrequired,seecomments)
      ApplicationCommentsPretreatparaffinsectionsbyheatingtissuein10mMcitratebuffer,pH6.0for1minathighpowerfollowedby9minatmediumpower;keeptheslidesfullyimmersedandmaintainthetemperatureatorjustbelowboiling;cooltheslidesfor20minatroomtemperaturepriortostaining.Variablesassociatedwithassayconditionswilldictatetheproperworkingdilution.

      RecommendedProtocolforImmunoblotting

      SolutionsandReagents
      ?TransferBuffer:25mMTrisbase,0.2Mglycine,20%methanol,pH8.5.
      ?SDSSampleBuffer:62.5mMTris-HCl,pH6.8,2%SDS,10%glycerol,50mMDTT,0.1%bromophenolblue.
      ?10XTBS(Tris-bufferedsaline):Toprepare1liter,24.2gTrisbase,80gNaCl,adjustpHto7.6withHCl.Dilute1:10foruse.
      ?BlockingBuffer:1XTBS,0.1%Tween?-20detergentwith5%non-fatdrymilk.
      ?PrimaryAntibodyDilutionBuffer:1XTBS,0.1%Tween?-20detergentwith5%BSA
      ?WashBuffer(TBST):1XTBS,0.1%Tween?-20detergent

      BlottingMembrane
      NitrocelluloseorPVDFmembranesmaybeused.

      ProteinBlotting
      1.Lysecellsbyadding100mlSDSSampleBufferandimmediatelyscrapethecellsofftheplateandtransfertheextracttoamicrofugetube.Keeponice.
      2.Sonicatefor2stoshearDNAandreducesampleviscosity.
      3.Heatsampleto95-100°Cfor5min.Coolonice.
      4.Microcentrifugefor5min.
      5.Load20mlontoSDS-PAGEgel(10cmx10cm).
      6.Electrotransfertonitrocellulosemembrane.

      Ascontrols,werecommendusing15mlofphosphorylatedandnonphosphorylatedC-6gliomacellextracts.

      MembraneBlocking,GelandAntibodyIncubations
      1.Aftertransfer,washmembranewith25mlTBSfor5minatroomtemperature.
      2.Incubatemembranein25mlofBlockingBufferfor1-3hatroomtemperatureorovernightat4°C.
      3.Wash3timesfor5mineachwith15mlTBST.
      4.Incubatemembraneandprimaryantibody(attheappropriatedilution)in10mlPrimaryAntibodyDilutionBufferwithgentleagitationovernightat4°C.
      5.Wash3timesfor5mineachwith15mlTBST.
      6.Incubatemembranewithconjugatedsecondaryantibodyattheappropriatedilutionin10mlBlockingBufferwithgentleagitationfor1hatroomtemperature.
      7.Washmembraneasinstep5.

      DetectionofProteins
      Chemiluminescence.
      BIOLOGicalInformation
      Immunogenasyntheticpeptide(TYDEYISFVPPPLDQEEMES)correspondingtoaminoacids341-360ofhumanp38MAPkinase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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