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Millipore/17-344 | H2A.X Phosphorylation Assay Kit (Flow Cytometry)/17-344/1 kit100 assays
  • Millipore/17-344 | H2A.X Phosphorylation Assay Kit (Flow Cytometry)/17-344/1 kit100 assays

Millipore/17-344 | H2A.X Phosphorylation Assay Kit (Flow Cytometry)/17-344/1 kit100 assays

價格: ￥4164.00 市場價: 6940.00
貨號: 17-344
品牌: Millipore
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    • Description
      CatalogueNumber17-344
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionH2A.XPhosphorylationAssayKit(FlowCytometry)
      OverviewPhosphorylationofthehistonevariantH2A.XisarapidandsensitiveresponsetodoublestrandDNAbreaks.TheH2A.XPhosphorylationAssayKit(Flowcytometry)isacellbasedassayformattedforflowcytometricdetectionoflevelsofphosphorylatedhistoneH2A.X.

      TheH2A.XPhosphorylationAssayKit(Flowcytometry)isacellbasedassayformattedforflowcytometricdetectionoflevelsofphosphorylatedHistoneH2A.X.Cellsareculturedinmicroplates,treatedwithagentsthatinduceDNAdamageorapoptosis,whichstimulatesH2A.Xphosphorylation.CellsarethenfixedandpermeABIlizedinpreparationforstaininganddetection.HistoneH2A.Xphosphorylatedatserine139isdetectedbytheadditionoftheanti-phospho-HistoneH2A.X,FITCconjugate.CellsarethenscannedinaflowcytometertoquantitatethenumberofcellsstainingpositiveforphosphorylatedHistoneH2A.X.
      ProductInformation
      Components
      • Anti-phosphoH2A.X,FITCconjugate
      • NormalMouseIgG,FITCconjugate(Cat.#12-487)
      • 16XFixationSolution
      • 10XPermeabilizationSolution
      • 10XWashSolution
      DetectionmethodFluorescent
      StorageandShippingInformation
      Applications
      ApplicationTheH2A.XPhosphorylationAssayKit(Flowcytometry)isacellbasedassayformattedforflowcytometricdetectionoflevelsofphosphorylatedHistoneH2A.X.
      KeyApplications
      • FlowCytometry
      BIOLOGicalInformation
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Afamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.
      GeneSymbol
      • H2AFX
      • H2A/X
      • H2A.X
      • H2AX
      • H2a/x
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P16104#VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation.
      SIZE:143aminoacids;15145Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.InteractswithnumerousproteinsrequiredforDNAdamagesignalingandrepairwhenphosphorylatedonSer-140.TheseincludeMDC1,TP53BP1,BRCA1andtheMRNcomplex,composedofMRE11A,RAD50,andNBN.InteractionwiththeMRNcomplexismediatedatleastinpartbyNBN.AlsointeractswithDHX9/NDHIIwhenphosphorylatedonSer-140.
      SUBCELLULARLOCATION:Nucleus.DEVELOPMENTALSTAGE:SynthesizedinG1aswellasinS-phase.
      DOMAIN:SwissProt:P16104The[ST]-QmotifconstitutesarecognitionsequenceforkinasesfromthePI3/PI4-kinasefamily.
      PTM:PhosphorylatedonSer-140(toformgamma-H2AFX)inresponsetoDNAdoublestrandbreaks(DSBs)generatedbyexogenousgenotoxicagentsandbystalledreplicationforks,andmayalsooccurduringmeioticrecombinationeventsandimmunoglobulinclassswitchinginlymphocytes.PhosphorylationcanextenduptoseveralthousandnucleosomesfromtheactualsiteoftheDSBandmaymarkthesurroundingchromatinforrecruitmentofproteinsrequiredforDNAdamagesignalingandrepair.Widespreadphosphorylationmayalsoservetoamplifythedamagesignaloraidrepairofpersistentlesions.PhosphorylationofSer-140inresponsetoionizingradiationismediatedbybothATMandPRKDCwhiledefectsinDNAreplicationinduceSer-140phosphorylationsubsequenttoactivationofATRandPRKDC.DephosphorylationofSer-140byPP2AisrequiredforDNADSBrepair.Inmeiosis,Ser-140phosphorylationmayoccuratsynaptonemalcomplexesduringleptoteneasanATM-dependentresponsetotheformationofprogrammedDSBsbySPO11.Ser-140phosphorylationmaysubsequentlyoccursatunsynapsedregionsofbothautosomesandtheXYbivalentduringzygotene,downstreamofATRandBRCA1activation.Ser-140phosphorylationmayalsoberequiredfortranscriptionalrepressionofunsynapsedchromatinandmeioticsexchromosomeinactivation(MSCI),wherebytheXandYchromosomescondenseinpachytenetoformtheheterochromaticXY-body.Duringimmunoglobulinclassswitchrecombinationinlymphocytes,Ser-140phosphorylationmayoccuratsitesofDNA-recombinationsubsequenttoactivationoftheactivation-inducedcytidinedeaminaseAICDA.&MonoubiquitinationofLys-120byRING1andRNF2/RING2complexgivesaspecifictagforepigenetictranscriptionalrepression(Bysimilarity).
      SIMILARITY:BelongstothehistoneH2Afamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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