Immunogen | KLH-conjugated,syntheticpeptidespanningaminoacids7-17(...GATISKKGFKK...)ofhumantestis/spermspecifichistoneH2B |
Host | Rabbit |
Specificity | histoneH2B,testisvariant |
SpeciesReactivity | |
SpeciesReactivityNote | Predictedtocross-reactwithratbasedonsequencehomology |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.Thisgeneisintronlessandencodesatestis/sperm-specificmemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement. |
GeneSymbol | - HIST1H2BA
- H2BFU
- bA317E16.3
- STBP
- TSH2B
|
PurificationMethod | Antiserum |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q96A08#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:127aminoacids;14167Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Transcribedexclusivelyintestis,andthecorrespondingproteinisalsopresentinmaturesperm. PTM:MonoubiquitinationofLys-122bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII. SIMILARITY:SwissProt:Q96A08##BelongstothehistoneH2Bfamily. |
MolecularWeight | Mr15kDa |