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Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL
  • Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

價(jià)格: ￥3576.00 市場價(jià): 5960.00
貨號: 07-596
品牌: Millipore
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    • Description
      CatalogueNumber07-596
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-HistoneH4(citrulline3)Antibody
      AlternateNames
      • Cit-H4R3
      • H4Cit3
      • HistoneH4,Arg3citrullinated
      BackgroundInformationHistoneH4(UniProtP62805;alsoknownasH4histonefamilymemberA,Histone1H4a)isencodedbytheHIST1H4A(alsoknownasH4/A,H4FA)gene(GeneID8359)inhuman.HistoneH4isoneofthemainhistoneproteinsthatcontributetothechromatinstructureinbothprokaryoticandeukaryoticcells.HistoneH4canundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses,includingmethylationandacetylationonlysineandarginineresidues,phosphorylationonserineandthreonineresidues,aswellasconversionArg3toacitrullineresiduebythepeptidylargininedeiminasePAD4-catalyzeddeiminationreaction,alsoknownascitrullination).HistoneH4Arg3citrullination(H4Cit3)weakenshistone-DNAinteractionasaresultofaconformationalchangeandareducedpositivechargeatthehistoneH4Nterminalend.BacterialinfectionisknowntostimulateH4Cit3inneutrophils,forminghighlydecondensedextracellularchromatinstructureNET(neutrophilextracellulartrap)thatcouldcaptureandkillmicroorganisms.Ontheotherhand,citrullinatedproteinsarerecognizedasnon-selfproteinsandsubsequentlyactivateimmunesystems.AfunctionalhaplotypeofthePADI4genehasbeenshowntobeassociatedwiththepathogenesisofrheumatoidarthritisthroughinductionoftheantigenicityofproteins.H4Cit3isinducedinresponsetoDNAdamagethroughthep53-PADI4pathway,resultinginupregulatedH4Cit3immunoreactivitylocalizedaroundfragmentednucleiinapoptoticcells.Inaddition,H4Cit3levelisnegativelycorrelatedwithp53proteinexpressionandtumoursizeinnon-smallcelllungcancer(NSCLC)tissues,indicatingacrucialroleofH4Cit3incarcinogenesis.
      ProductInformation
      FormatAffinityPurified
      PresentationPurifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationAnti-HistoneH4(Citrulline3)Antibody,Cat.No.07-596,isahighlyspecificrabbitpolyclonalantibodythattargetshistoneH4citrullination(H4Cit3)andhasbeentestedinChromatinImmunoprecipitation(ChIP),Immunocytochemistry,Immunohistochemistry,andWesternBlotting.
      KeyApplications
      • ChromatinImmunoprecipitation(ChIP)
      • Immunocytochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelot(3μLperChIP)detectedhistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsthathadbeentreatedwithβ-estradiolforonehouraftera3-dayserum-starvationperiod.

      ImmunocytochemistryAnalysis:A1:500dilutiondetectedfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullinationinionomycin-stimulatedprimaryhumanneutrophils(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics).

      ImmunohistochemistryAnalysis:A1:250dilutionfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics).

      ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelotdetectedatime-dependenthistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsfollowingestradioltreatment(Wang,Y.,etal.(2004).Science.306(5694):279-283).

      ImmunocytochemistryAnalysis:ArepresentativelotdetectedenhancednuclearH4/H2AAr3citrullinationinHEK293TandU2OScellsuponexogenousPAD4expressionorDNAdamageinduction(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

      ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeABIlizedcasein-elicitedperitonealneutrophilscellsfromwild-type,butnotPad4-knockoutmice(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043).

      ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeabilizedinfiltratinglungleukocytesisolatedfromwild-type,butnotPad4-knockoutmicefollowinginfluenzainfection(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043).

      ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in4%paraformaldehyde-fixed,1%NP-40/0.5%TritonX-100-permeabilizedperipheralbloodcellsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

      ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)insynovialcellsandextracellulartraps(NETs)flushedoutoftheanklejointsofwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

      ImmunocytochemistryAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4(H4Cit3)Arg3citrullinationin3.7%paraformaldehyde-fixed,0.2%NP-40/0.1%TritonX-100-permeabilizedHL-60cells(Wang,Y.,etal.(2004).Science.306(5694):279-283).
      ImmunohistochemistryAnalysis:Arepresentativelotdetectedgammairradiation-inducednuclearH4/H2AAr3citrullinationinthymustissuesectionsfromwild-type,butnotPad4-knockoutmice(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

      ImmunohistochemistryAnalysis:ArepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice10dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

      WesternBlottingAnalysis:ArepresentativelotdetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellasDNAdamage-inducedcellularH4andH2AAr3citrullination(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

      WesternBlottingAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4knockdownbyshRNAtreatmentgreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Wang,Y.,etal.(2004).Science.306(5694):279-283).
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,syntheticpeptidecorrespondingtoaminoacids1-10(SG[cit]GKGGKGL-C)ofhumanhistoneH4(Met1removed),where[cit]denotescitrullinatedArg3.AC-terminalcysteinewasaddedforconjugationpurposes.
      EpitopeN-terminus
      ConcentrationPleaserefertolotspecificdatasheet.
      HostRabbit
      SpecificityThisrabbitpolyclonalantibodydetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellascalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4(peptidylargininedeiminase4)knockdowngreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Tanikawa,C.,etal.(2012).Nat.Commun.3:676;Wang,Y.,etal.(2004).Science.306(5694):279-283).
      SpeciesReactivity
      • Human
      • Mouse
      SpeciesReactivityNoteHuman,Mouse.Predictedtoreactwithabroadrangeofspeciesbasedon100%sequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • HIST1H4A
      • H4/A
      • ;H4FA
      • HIST1H4B
      • H4/I
      • ;H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      • HIST1H4A
      • H4/A
      Modifications
      • Citrullination
      PurificationMethodAffinitypurified.
      UniProtNumber
      MolecularWeight~12kDaobserved.11.24/11.37kDa(H4with/withoutMet1removal)calculated.Uncharacterizedbandsmaybeobservedinsomelysate(s).
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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