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Millipore/07-1232 | Anti-Notch 1 Antibody, NT/07-1232/200 µL
  • Millipore/07-1232 | Anti-Notch 1 Antibody, NT/07-1232/200 µL

Millipore/07-1232 | Anti-Notch 1 Antibody, NT/07-1232/200 µL

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貨號: 07-1232
品牌: Millipore
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    • Description
      CatalogueNumber07-1232
      Replaces04-1046
      DescriptionAnti-Notch1Antibody,NT
      AlternateNames
      • Neurogeniclocusnotchhomologprotein1precursor
      • Notch(Drosophila)homolog1(translocation-associated)
      • Notch1
      • Notchhomolog1,translocation-associated(Drosophila)
      • Translocation-associatednotchproteinTAN-1
      • Neurogeniclocusnotchhomologprotein1
      BackgroundInformationNotchisafamilyofsingle-passtransmembranereceptorproteinsthataresynthesizedintheendoplasmicreticulumasaninactiveformandareproteolyticallycleavedonanextracellularsitebyafurin-likeconvertase(S1cleavage)inthetrans-golginetworkaftertherecognitionoftheRQRRsequencebeforeitreachestheplasmamembraneasheterodimerstoyieldanactive,ligand-accessIBLeform.TheNotchfamilyiscomprisedof4members(1-4)whoseligandsincludetheDeltaandJaggedfamilyofligands.TheseligandscauseproteolysisofNotchtoliberatetheintracellulardomain.CleavageresultsinaC-terminalfragmentN(TN)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleaved(S2cleavage)byTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcalledNotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilin-dependentgamma-secretasebetweengly1743andval1744(S3cleavage)toreleasetheintracellulardomain(NICD)fromthemembrane.ThatdomaintranslocatestothenucleusandinitiatestranscriptioneventsbybindingtheDNAbindingproteinCSL.Thenotchfamilymembersareinvolvedcelldifferentiationanddevelopment.
      ProductInformation
      FormatSerum
      Control
      • JurkateCellLysates.Infetaltissues,usespleen,brainstemandlung.Inadulttissuesusemainlylymphoidtissues.
      PresentationRabbitpolyclonalIgGseruminbuffercontaining0.02MPotassiumPhosphate,0.15MNaCl,pH7.2,containing0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationAnti-Notch1Antibody,N-TerminusisaRabbitPolyclonalAntibodyfordetectionofNotch1alsoknownasNeurogeniclocusnotchhomologprotein1precursor&hasbeenvalidatedinELISA,WB,IHC(P).
      KeyApplications
      • ELISA
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      ApplicationNotesELISA:
      Apreviouslotofthisantibodywasdilutedto1:20,000-1:60,000andusedinastandardsandwichELISAassayagainstthepeptideimmunogen.

      Immunohistochemistry(paraffin):
      Representativetestingfromapreviouslot.
      OptimalStainingofNotch-1WithCitratepH6.0EpitopeRetrieval:UterineCancer

      ELISA:Apreviouslotofthisantibodywasdilutedto1:20,000-1:60,000andusedinastandardsandwichELISAassayagainstthepeptideimmunogen.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidefromtheN-terminalsequenceofthecleavedNintracellulardomain(NICD)humanNotch1.
      EpitopeCleavedN-Terminus
      HostRabbit
      SpecificityNotch1,cleavedNterminal.Onlythecleavedintracellular(activated)formisdetected.NoreactivityisdetectedagainstmouseN2,N3orN4.Thisepitopeisexposedonlyaftergammasecretasecleavageandisnotaccessibleintheuncleavedform.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      SpeciesReactivityNoteHumanandmouse.Theantibodyisalsoexpectedtoreactwithratduetoimmunogensequencehomology.Otherspecieshavenotbeentested.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.
      GeneSymbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.Maybeimportantforfolliculardifferentiationandpossiblycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity).
      SIZE:2556aminoacids;272500Da
      MolecularWeight~80kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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