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Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg
  • Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

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    • Description
      CatalogueNumber05-806
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-phospho-HistoneH3(Ser10)Antibody,clone3H10
      AlternateNames
      • H3histone,family3A
      • H3S10P
      • HistoneH3(phosphoS10)
      • H3histone,family3A
      • H3histone,family3B
      • H3histone,family3B(H3.3B)
      BackgroundInformationHistoneH3isoneofthefivemainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongN-terminaltail,H3isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.TheN-terminaltailofhistoneH3protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.
      ProductInformation
      FormatPurified
      Control
      • UV-treated293cellextracts,UV-treatedHeLacellextracts,breastcancertissue,HEPG2cellextracts.
      PresentationPurifiedmouseIgG1kinbuffercontaining0.1MTris-glycine,pH7.4,0.15MNaCland0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2to8°Cfromdateofreceipt.

      HandlingRecommendations:
      Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationAnti-phospho-HistoneH3(Ser10)Antibody,clone3H10isamousemonoclonalantibodyfordetectionofHistoneH3phosphorylatedatserine10.ThispurifiedmAb,alsoknownasH3S10p,ispublishedinpeerreviewedjournalsandhasbeenvalidatedinFC,ICC,IF,PIA,WB,Mplex.
      KeyApplications
      • FlowCytometry
      • Immunocytochemistry
      • Immunofluorescence
      • PeptideInhibitionAssay
      • WesternBlotting
      • Multiplexing
      ApplicationNotesFlowCytometry:
      ThisantibodyhasbeenreportedbyanindependentlaboratorytodetectphosphorylatedhistoneH3usingflowcytometry.

      Beadlyte?Histone-PeptideSpecificityAssay:
      1:1,000to1:81,000dilutionsofapreviouslotwereincubatedwithhistoneH3peptidescontainingvariousmodificationsconjugatedtoLuminex?microspheres.(FigureB).Onlythepeptidecontainingphospho-serine10wasdetected.


      Immunocytochemistry:
      0.2μg/mLofapreviouslotshowedpositivechromosomeimmunostainingformitoticA431andHeLacellsfixedwith95%ethanoland5%aceticacidandpermeabilizedwith0.1%Triton?X-100.

      PeptideInhibitionAnalysis:
      DetectionofhistoneH3inimmunoblotsofcolcemidtreatedHeLaacidextractsbyanti-phospho-HistoneH3(Ser10)wasdiminishedby10μMofhistoneH3peptidecontainingphospho-serine10,butnotbypeptidescontainingphospho-serine28oranunmodifiedhistoneH3sequence(FigureC).

      Immunofluorescence:
      BIOLOGicalInformation
      ImmunogenAproprietaryimmunogenbasedonapeptidesequencecontainingphospho-serinecorrespondingtoresidue10ofhumanhistoneH3.
      Clone3H10
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityHistoneH3phosphorylatedatSer10.
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.
      Broadspeciescross-reactivityisexpected.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH3family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.ThisgeneislocatedseparatelyfromtheotherH3genesthatareinthehistonegeneclusteronchromosome6p22-p21.3.
      GeneSymbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q16695#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:136aminoacids;15508Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      TISSUESPECIFICITY:Expressedintesticularcells.
      DEVELOPMENTALSTAGE:ExpressedduringSphase,thenexpressionstronglydecreasesascelldivisionslowsdownduringtheprocessofdifferentiation.
      PTM:Acetylationisgenerallylinkedtogeneactivation.AcetylationonLys-10impairsmethylationatArg-9.AcetylationonLys-19andLys-24favorsmethylationatArg-18(Bysimilarity).&CitrullinationatArg-9and/orArg-18byPADI4impairsmethylationandrepressestranscription(Bysimilarity).&AsymmetricdimethylationatArg-18byCARM1islinkedtogeneactivation.SymmetricdimethylationatArg-9byPRMT5islinkedtogenerepression(Bysimilarity).&MethylationatLys-5,Lys-37andLys-80arelinkedtogeneactivation.MethylationatLys-5facilitatessubsequentacetylationofH3andH4.MethylationatLys-80isassociatedwithDNAdouble-strandbreak(DSB)responsesandisaspecifictargetforTP53BP1.MethylationatLys-10andLys-28arelinkedtogenerepression.MethylationatLys-10isaspecifictargetforHP1proteins(CBX1,CBX3andCBX5)andpreventssubsequentphosphorylationatSer-11andacetylationofH3andH4.MethylationatLys-5andLys-80requirepreliminarymonoubiquitinationofH2Bat"Lys-120".MethylationatLys-10andLys-28areenrichedininactiveXchromosomechromatin(Bysimilarity).&PhosphorylatedatThr-4byGSG2/haspinduringprophaseanddephosphorylatedduringanaphase.Atcentromeres,specificallyphosphorylatedatThr-12fromprophasetoearlyanaphase.PhosphorylatedatSer-11duringthewholemitosis.PhosphorylationatSer-11,whichislinkedtogeneactivation,preventsmethylationatLys-10butfacilitatesacetylationofH3andH4.PhosphorylatedatSer-29byMLTKisoform1,RPS6KA5orAURKBduringmitosisoruponultravioletBirradiation(Bysimilarity).&Phosphorylationat"Ser-11"iscrucialforchromosomecondensationandcell-cycleprogressionduringmitosisandmeiosis.Inadditionphosphorylationat"Ser-11"isimportantduringinterphasebecauseitenablesthetranscriptionofgenesfollowingexternalstimulation,likestressorgrowthfactors.Phosphorylationat"Ser-11"isalsoanessentialregulatorymechanismforneoplasticcelltransformation.Phosphorylationat"Ser-11"byAURKB/Aurora-BmediatesthedissociationofHP1proteins(CBX1,CBX3andCBX5)fromheterochromatin.&Ubiquitinated(Bysimilarity).
      SIMILARITY:SwissProt:Q16695##BelongstothehistoneH3family.
      MolecularWeight17kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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