Control | - UntreatedA-431(negativecontrol)andEGFtreatedA-431(positivecontrol)wholecelllysates.
IncludedPositiveAntigenControl:Catalog#12-302,EGF-stimulatedA431celllysateisprovidedasafreepositiveantigencontrolforwesternimmunoblotting.Aliquotasdesired,refreezeimmediately,andstoreat-20°C.Thelysateisstablefor6monthsat-20°C.Beforeuse,add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforimmunoblotanalysis.
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