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Millipore/04-1530 | Anti-MDM2 Antibody, clone 3G9/04-1530/100 µg
  • Millipore/04-1530 | Anti-MDM2 Antibody, clone 3G9/04-1530/100 µg

Millipore/04-1530 | Anti-MDM2 Antibody, clone 3G9/04-1530/100 µg

價(jià)格: ￥3696.00 市場(chǎng)價(jià): 6160.00
貨號(hào): 04-1530
品牌: Millipore
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    • Description
      CatalogueNumber04-1530
      DescriptionAnti-MDM2Antibody,clone3G9
      AlternateNames
      • Doubleminute2protein
      • Mdm2p53bindingproteinhomolog(mouse)
      • Mdm2,transformed3T3celldoubleminute2,p53bindingprotein
      • Mdm2,transformed3T3celldoubleminute2,p53bindingprotein(mouse)
      • OncoproteinMdm2
      • doubleminute2,humanhomologof
      • p53-bindingprotein
      • mousedoubleminute2homolog
      • mousedoubleminute2,humanhomologof
      • p53-bindingprotein
      • p53-bindingproteinMdm2
      • ubiquitin-proteinligaseE3Mdm2
      BackgroundInformationMDM2isover-expressedinmanytumors.Itsprincipalfunctionistheubiquitinationanddegradationofp53tumorsuppressorprotein.ThismonoclonalantibodyalsorecognizesapeptideepitopearoundThr-216ofmurineMDM2whenThr-216isunphosphorylated.SMP14alsocrossreactswithsomecytokeratins(6,14&16).Thisisonlyaproblemwhenworkingwithcertainepithelialcellsandnotfibroblasts.
      ProductInformation
      FormatPurified
      Control
      • MCF-7celllysate
      PresentationPurifiedmousemonoclonalIgGκinbuffercontaining0.1MTris-Glycine(pH7.4,150mMNaCl)with0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationAnti-MDM2Antibody,clone3G9isaMouseMonoclonalAntibodyfordetectionofMDM2alsoknownasDoubleminute2proteinorMdm2p53bindingproteinhomolog&hasbeenvalidatedinWB,IP,ICC&IHC.
      KeyApplications
      • WesternBlotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesImmunoprecipitationAnalysis:Arepresentativelotwasusedbyanindependentlaboratoryforthisapplication.(Changgong,L.,etal.(2002).MolecularandCellularBIOLOGy.22(21):7562-7571.)
      BiologicalInformation
      ImmunogenHis-taggedrecombinantproteincorrespondingtohumanMDM2.
      EpitopeUnknown
      Clone3G9
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizesMDM2.
      SpeciesReactivity
      • Human
      • Mouse
      SpeciesReactivityNoteDemonstratedtoreactwithhuman.Predictedtoreactwithmousebasedon100%sequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisatargetgeneofthetranscriptionfactortumorproteinp53.Theencodedproteinisanuclearphosphoproteinthatbindsandinhibitstransactivationbytumorproteinp53,aspartofanautoregulatorynegativefeedbackloop.Overexpressionofthisgenecanresultinexcessiveinactivationoftumorproteinp53,diminishingitstumorsuppressorfunction.ThisproteinhasE3ubiquitinligaseactivity,whichtargetstumorproteinp53forproteasomaldegradation.Thisproteinalsoaffectsthecellcycle,apoptosis,andtumOrigenesisthroughinteractionswithotherproteins,includingretinoblastoma1andribosomalproteinL5.Morethan40differentalternativelysplicedtranscriptvariantshavebeenisolatedfrombothtumorandnormaltissues.[providedbyRefSeq].
      GeneSymbol
      • MDM2
      • hdm2
      • HDMX
      • Hdm2
      • HDM2
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:InhibitsTP53/p53-andTP73/p73-mediatedcellcyclearrestandapoptosisbybindingitstranscriptionalactivationdomain.FunctionsasaubiquitinligaseE3,inthepresenceofE1andE2,towardp53anditself.Permitsthenuclearexportofp53andtargetsitforproteasome-mediatedproteolysis.

      SIZE:491aminoacids;55233Da

      SUBUNIT:Bindsp53,p73,ARF(P14),ribosomalproteinL5andspecificallytoRNA.Caninteractalsowithretinoblastomaprotein(RB),E1A-associatedproteinEP300andtheE2F1transcriptionfactor.Formsaternarycomplexwithp53andWWOX.InteractswithUSP7andTBRG1.IsoformMdm2-FdoesnotinteractwithTP53/p53.Interactswith,andubiquitinatesHIV-1Tat.

      SUBCELLULARLOCATION:Nucleus,nucleoplasm.Cytoplasm.Nucleus,nucleolus.Note=Expressedpredominantlyinthenucleoplasm.InteractionwithARF(P14)resultsinthelocalizationofbothproteinstothenucleolus.ThenucleolarlocalizationsignalsinbothARF(P14)andMDM2maybenecessarytoallowefficientnucleolarlocalizationofbothproteins.

      TISSUESPECIFICITY:Ubiquitous.IsoformMdm2-A,isoformMdm2-B,isoformMdm2-C,isoformMdm2-D,isoformMdm2-E,isoformMdm2-FandisoformMdm2-Gareobservedinarangeofcancersbutabsentinnormaltissues.

      DOMAIN:RegionIissufficientforbindingp53andinhibitingitsG1arrestandapoptosisfunctions.Italsobindsp73andE2F1.RegionIIcontainsmostofacentralacidicregionrequiredforinteractionwithribosomalproteinL5andaputativeC4-typezincfinger.TheRINGfingerdomainwhichcoordinatestwomoleculesofzincinteractsspecificallywithRNAwhetherornotzincispresentandmediatesthehetero-oligomerizationwithMDM4.ItisalsoessentialforitsubiquitinligaseE3activitytowardp53anditself.

      PTM:PhosphorylatedinresponsetoionizingrADIationinanATM-dependentmanner.

      DISEASE:Seemstobeamplifiedincertaintumors(includingsofttissuesarcomas,osteosarcomasandgliomas).Ahigherfrequencyofsplicevariantslackingp53bindingdomainsequenceswasfoundinlate-stageandhigh-gradeovarianandbladdercarcinomas.Fourofthesplicevariantsshowlossofp53binding.

      SIMILARITY:BelongstotheMDM2/MDM4family.&Contains1RanBP2-typezincfinger.&Contains1RING-typezincfinger.&Contains1SWIBdomain.

      MISCELLANEOUS:MDM2RINGfingermutationsthatfailedtoubiquitinatep53invitrodidnottargetp53fordegradationwhenexpressedincells.
      MolecularWeight~75kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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