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Millipore/04-1062 | Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal/04-1062/100 µL
  • Millipore/04-1062 | Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal/04-1062/100 µL

Millipore/04-1062 | Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal/04-1062/100 µL

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貨號(hào): 04-1062
品牌: Millipore
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    • Description
      CatalogueNumber04-1062
      DescriptionAnti-GFAP(NT)Antibody,cloneEPR1034Y,rabbitmonoclonal
      AlternateNames
      • glialfibrillaryacidicprotein
      BackgroundInformationGlialfibrillaryacidicproteinisaclass-IIIintermediatefilament.GFAPisthemainconstituentofintermediatefilamentsinastrocytesandservesasacellspecificMarkerthatdistinguishesdifferentiatedastrocytesfromotherglialcellsduringthedevelopmentofthecentralnervoussystem.
      ProductInformation
      FormatUnpurified
      Control
      • HumanandRatbrainlysates
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.DetectGFAP(N-term)usingthisAnti-GFAP(N-term)Antibody,cloneEPR1034Y,RabbitvalidatedforuseinWB,IH(P),IC&IP.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      ApplicationNotesImmunohistochemistryAnalysis:A1:250-500dilutionfromapreviouslotdetectedGFAPinhumanbrainandhumangliomatissue.
      ImmunocytochemistryAnalysis:A1:100-250dilutionfromapreviouslotwasusedinIC.
      ImmunoprecipitationAnalysis:A1:40dilutionfromapreviouslotwasusedinIP.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoresiduesintheN-terminusofhumanGFAP.
      EpitopeN-terminus
      CloneEPR1034Y
      HostRabbit
      SpecificityThisantibodyrecognizesGFAPatandaroundtheN-terminus.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Alternativesplicingresultsinmultipletranscriptvariantsencodingdistinctisoforms.
      GeneSymbol
      • FLJ45472
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.
      SUBUNITSTRUCTURE:InteractswithSYNMBysimilarity.Isoform3interactswithPSEN1(viaN-terminus).
      SUBCELLULARLOCATION:Cytoplasm.Note:Associatedwithintermediatefilaments.
      TISSUESPECIFICITY:Expressedincellslackingfibronectin.
      INVOLVEMENTINDISEASE:DefectsinGFAPareacauseofAlexanderdisease(ALEXD)[MIM:203450].Alexanderdiseaseisararedisorderofthecentralnervoussystem.ItisaprogressiveleukoencephalopathywhosehallmarkisthewidespreadaccumulationofRosenthalfiberswhicharecytoplasmicinclusionsinastrocytes.Themostcommonformaffectsinfantsandyoungchildren,andischaracterizedbyprogressivefailureofcentralmyelination,usuallyleADIngtodeathusuallywithinthefirstdecade.InfantswithAlexanderdiseasedevelopaleukoencephalopathywithmacrocephaly,seizures,andpsychomotorretardation.Patientswithjuvenileoradultformstypicallyexperienceataxia,bulbarsignsandspasticity,andamoreslowlyprogressivecourse.
      SEQUENCESIMILARITIES:Belongstotheintermediatefilamentfamily.
      MolecularWeight~50kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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