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Millipore/04-1045 | Anti-Caspase-6 (Pro) Antibody, clone EP1325Y, rabbit monoclonal/04-1045/100 µL
  • Millipore/04-1045 | Anti-Caspase-6 (Pro) Antibody, clone EP1325Y, rabbit monoclonal/04-1045/100 µL

Millipore/04-1045 | Anti-Caspase-6 (Pro) Antibody, clone EP1325Y, rabbit monoclonal/04-1045/100 µL

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貨號: 04-1045
品牌: Millipore
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    • Description
      CatalogueNumber04-1045
      DescriptionAnti-Caspase-6(Pro)Antibody,cloneEP1325Y,rabbitmonoclonal
      AlternateNames
      • CASP-6
      BackgroundInformationCaspasesareafamilyofcytosolicaspartate-specificcysteineproteasesinvolvedintheinitiationandexecutionofapoptosis.Caspase-6isamajorapoptoticexecutioner(1,2).Itexistsasapro-domainproteinandiscleavedbycaspase-3intotwoactivesubunits,an18kDaanda10kDasubunit(3).Caspase-6cleavesamongmanydifferentintracellularproteins,nuclearlamins.Laminsplayasignificantroleinmaintainingtheintegrityofthecellmembrane,andtheircleavageresultsinsubsequentcellularbreakdownanddeath(4).
      ProductInformation
      FormatUnpurified
      Control
      • MCF-7celllysates
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      • Immunoprecipitation
      • FlowCytometry
      • Immunocytochemistry
      ApplicationNotesImmunohistochemistryAnalysis:A1:100-250dilutionfromapreviouslotdetectedpro-Caspase6inhumanbreastcarcinomatissueandHeLacelllysate.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoresiduesofhumanpro-Caspase-6.
      CloneEP1325Y
      HostRabbit
      SpecificityThisantibodyrecognizespro-Caspase6.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      SpeciesReactivityNoteProventoreactinhuman.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteinisprocessedbycaspases7,8and10,andisthoughttofunctionasadownstreamenzymeinthecaspaseactivationcascade.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodedifferentisoforms.[providedbyRefSeq].
      GeneSymbol
      • CASP-6
      • MCH2
      • EC3.4.22.59
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Cleavespoly(ADP-ribose)polymeraseinvitro,aswellaslamins.Overexpressionpromotesprogrammedcelldeath.

      CATALYTICACTIVITY:StrictrequirementforAspatpositionP1andhasapreferredcleavagesequenceofVal-Glu-His-Asp-|-.

      ENZYMEREGULATION:ActivationissuppressedbyphosphorylationatSer-257.Ref.5

      SUBUNITSTRUCTURE:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya18kDa(p18)anda11kDa(p11)subunit.

      SUBCELLULARLOCATION:Cytoplasm.

      PTM:Cleavagesbycaspase-3,caspase-8or-10generatethetwoactivesubunits.

      SEQUENCESIMILARITY:BelongstothepeptidaseC14Afamily.

      MolecularWeight~35kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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